Anti-actin antibodies. Detection and quantitation of total and skeletal muscle actin in human plasma using a competitive ELISA.
J Immunol Methods
; 99(1): 129-35, 1987 May 04.
Article
em En
| MEDLINE
| ID: mdl-3571989
ABSTRACT
A competitive ELISA has been used to titrate skeletal muscle and total actins in human plasma. Specific antibodies directed against the variable N-terminal 1-7 sequence and conserved sequences respectively were used. The N-terminus of actin appears to be accessible in native and brevin-complexed actins. The skeletal muscle actin isoform represents about 1% of the total circulating actin (mean 50 micrograms/ml plasma), but is markedly increased after severe muscle tissue injuries.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Actinas
/
Proteínas dos Microfilamentos
/
Anticorpos
/
Músculos
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Adult
/
Humans
/
Middle aged
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1987
Tipo de documento:
Article