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Mapping immunogenic epitopes of an adhesin-like protein from Methanobrevibacter ruminantium M1 and comparison of empirical data with in silico prediction methods.
Khanum, Sofia; Carbone, Vincenzo; Gupta, Sandeep K; Yeung, Juliana; Shu, Dairu; Wilson, Tania; Parlane, Natalie A; Altermann, Eric; Estein, Silvia M; Janssen, Peter H; Wedlock, D Neil; Heiser, Axel.
Afiliação
  • Khanum S; AgResearch, Palmerston North, New Zealand. sofia.khanum@agresearch.co.nz.
  • Carbone V; AgResearch, Palmerston North, New Zealand.
  • Gupta SK; AgResearch, Palmerston North, New Zealand.
  • Yeung J; AgResearch, Palmerston North, New Zealand.
  • Shu D; AgResearch, Palmerston North, New Zealand.
  • Wilson T; AgResearch, Palmerston North, New Zealand.
  • Parlane NA; AgResearch, Palmerston North, New Zealand.
  • Altermann E; AgResearch, Palmerston North, New Zealand.
  • Estein SM; Riddet Institute, Massey University, Palmerston North, New Zealand.
  • Janssen PH; Centro de Investigación Veterinaria de Tandil (CIVETAN), UNCPBA-CONICET-CICPBA, Facultad de Ciencias Veterinarias, Campus Universitario, 7000, Tandil, Argentina.
  • Wedlock DN; AgResearch, Palmerston North, New Zealand.
  • Heiser A; AgResearch, Palmerston North, New Zealand.
Sci Rep ; 12(1): 10394, 2022 06 21.
Article em En | MEDLINE | ID: mdl-35729277
In silico prediction of epitopes is a potentially time-saving alternative to experimental epitope identification but is often subject to misidentification of epitopes and may not be useful for proteins from archaeal microorganisms. In this study, we mapped B- and T-cell epitopes of a model antigen from the methanogen Methanobrevibacter ruminantium M1, the Big_1 domain (AdLP-D1, amino acids 19-198) of an adhesin-like protein. A series of 17 overlapping 20-mer peptides was selected to cover the Big_1 domain. Peptide-specific antibodies were produced in mice and measured by ELISA, while an in vitro splenocyte re-stimulation assay determined specific T-cell responses. Overall, five peptides of the 17 peptides were shown to be major immunogenic epitopes of AdLP-D1. These immunogenic regions were examined for their localization in a homology-based model of AdLP-D1. Validated epitopes were found in the outside region of the protein, with loop like secondary structures reflecting their flexibility. The empirical data were compared with epitope predictions made by programmes based on a range of algorithms. In general, the epitopes identified by in silico predictions were not comparable to those determined empirically.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adesinas Bacterianas / Methanobrevibacter Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Nova Zelândia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adesinas Bacterianas / Methanobrevibacter Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Nova Zelândia