Antibody response in man following a small intradermal inoculation with Coxiella burnetii phase I vaccine.
Acta Virol
; 23(1): 73-81, 1979 Jan.
Article
em En
| MEDLINE
| ID: mdl-35962
ABSTRACT
A small inoculum (0.2 microgram) of phase I Coxiella burnetii vaccine given to individuals previously sensitized to CO burnetii elicited a positive skin reaction and a strong IgM phase I antibody response as determined by microagglutination, complement fixation and microimmunofluorescence tests. A similar inoculum administered to nonsensitized individuals did not elitic a skin reaction nor stimulate a recognizable antibody response. Serum from one of these sensitized and skin tested individuals was fractionated by gel filtration methods. The serum and serum fractions were titrated in a mouse seroprotection test using primary chicken embryo cell culture plaque technique as the assay procedure. Results of the mouse seroprotection test indicated that most of the protective activity of the serum was associated with the IgM fraction and that phase I IgM antibody suppressed the growth of C. burnetii in mouse spleen when mixed with the rickettsial suspension prior to inoculation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vacinas Bacterianas
/
Coxiella
/
Anticorpos Antibacterianos
Limite:
Adult
/
Humans
/
Male
Idioma:
En
Revista:
Acta Virol
Ano de publicação:
1979
Tipo de documento:
Article