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Deep mutational scanning identifies SARS-CoV-2 Nucleocapsid escape mutations of currently available rapid antigen tests.
Frank, Filipp; Keen, Meredith M; Rao, Anuradha; Bassit, Leda; Liu, Xu; Bowers, Heather B; Patel, Anamika B; Cato, Michael L; Sullivan, Julie A; Greenleaf, Morgan; Piantadosi, Anne; Lam, Wilbur A; Hudson, William H; Ortlund, Eric A.
Afiliação
  • Frank F; Department of Biochemistry, Emory University School of Medicine, Emory University, Atlanta, GA 30322, USA; The Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA 30322, USA. Electronic address: ffrank2@emory.edu.
  • Keen MM; Department of Biochemistry, Emory University School of Medicine, Emory University, Atlanta, GA 30322, USA; The Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA 30322, USA.
  • Rao A; The Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA 30322, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA; Center for Childhood Infections and Vaccines of Children's Healthcare of Atlanta, Emory University, Atlanta, GA 30
  • Bassit L; The Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA 30322, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA; Center for Childhood Infections and Vaccines of Children's Healthcare of Atlanta, Emory University, Atlanta, GA 30
  • Liu X; Department of Biochemistry, Emory University School of Medicine, Emory University, Atlanta, GA 30322, USA.
  • Bowers HB; The Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA 30322, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA; Center for Childhood Infections and Vaccines of Children's Healthcare of Atlanta, Emory University, Atlanta, GA 30
  • Patel AB; Department of Biochemistry, Emory University School of Medicine, Emory University, Atlanta, GA 30322, USA.
  • Cato ML; Department of Biochemistry, Emory University School of Medicine, Emory University, Atlanta, GA 30322, USA.
  • Sullivan JA; The Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA 30322, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA.
  • Greenleaf M; The Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA 30322, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA.
  • Piantadosi A; Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA; Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, GA 30322, USA.
  • Lam WA; The Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA 30322, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA; Wallace H. Coulter Department of Biomedical Engineering, Emory University, Georgia Institute of Technology, Atlant
  • Hudson WH; Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322, USA; Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA. Electronic address: william.hudson@bcm.edu.
  • Ortlund EA; Department of Biochemistry, Emory University School of Medicine, Emory University, Atlanta, GA 30322, USA; The Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA 30322, USA. Electronic address: eortlun@emory.edu.
Cell ; 185(19): 3603-3616.e13, 2022 09 15.
Article em En | MEDLINE | ID: mdl-36084631
ABSTRACT
The effects of mutations in continuously emerging variants of SARS-CoV-2 are a major concern for the performance of rapid antigen tests. To evaluate the impact of mutations on 17 antibodies used in 11 commercially available antigen tests with emergency use authorization, we measured antibody binding for all possible Nucleocapsid point mutations using a mammalian surface-display platform and deep mutational scanning. The results provide a complete map of the antibodies' epitopes and their susceptibility to mutational escape. Our data predict no vulnerabilities for detection of mutations found in variants of concern. We confirm this using the commercial tests and sequence-confirmed COVID-19 patient samples. The antibody escape mutational profiles generated here serve as a valuable resource for predicting the performance of rapid antigen tests against past, current, as well as any possible future variants of SARS-CoV-2, establishing the direct clinical and public health utility of our system.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: SARS-CoV-2 / COVID-19 Limite: Animals / Humans Idioma: En Revista: Cell Ano de publicação: 2022 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: SARS-CoV-2 / COVID-19 Limite: Animals / Humans Idioma: En Revista: Cell Ano de publicação: 2022 Tipo de documento: Article