Substantial gradient mitigation in simulated large-scale bioreactors by optimally placed multiple feed points.
Biotechnol Bioeng
; 119(12): 3549-3566, 2022 Dec.
Article
em En
| MEDLINE
| ID: mdl-36110051
ABSTRACT
The performance of large-scale stirred tank and bubble column bioreactors is often hindered by insufficient macromixing of feeds, leading to heterogeneities in pH, substrate, and oxygen, which complicates process scale-up. Appropriate feed placement or the use of multiple feed points could improve mixing. Here, theoretically optimal placement of feed points was derived using one-dimensional diffusion equations. The utility of optimal multipoint feeds was evaluated with mixing, pH control, and bioreaction simulations using three-dimensional compartment models of four industrially relevant bioreactors with working volumes ranging from 8 to 237 m3 . Dividing the vessel axially in equal-sized compartments and locating a feed point or multiple feed points symmetrically in each compartment reduced the mixing time substantially by more than a minute and mitigated gradients of pH, substrate, and oxygen. Performance of the large-scale bioreactors was consequently restored to ideal, homogeneous reactor performance oxygen consumption and biomass yield were recovered and the phenotypical heterogeneity of the biomass population was diminished.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxigênio
/
Reatores Biológicos
Idioma:
En
Revista:
Biotechnol Bioeng
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Finlândia