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Fabricating a multi-component microfluidic system for exercise-induced endothelial cell mechanobiology guided by hemodynamic similarity.
Na, Jing-Tong; Wang, Yan-Xia; Li, Yong-Jiang; Wang, Yu; Liu, Bo; Qin, Kai-Rong.
Afiliação
  • Na JT; School of Biomedical Engineering, Faculty of Medicine, Dalian University of Technology, Dalian 116024, China.
  • Chun-Dong Xue; School of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, Dalian 116024, China.
  • Wang YX; School of Rehabilitation Medicine, Weifang Medical University, Weifang 261053, China.
  • Li YJ; School of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, Dalian 116024, China.
  • Wang Y; School of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, Dalian 116024, China.
  • Liu B; School of Biomedical Engineering, Faculty of Medicine, Dalian University of Technology, Dalian 116024, China.
  • Qin KR; School of Biomedical Engineering, Faculty of Medicine, Dalian University of Technology, Dalian 116024, China; School of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, Dalian 116024, China. Electronic address: krqin@dlut.edu.cn.
Talanta ; 253: 123933, 2023 Feb 01.
Article em En | MEDLINE | ID: mdl-36113333
Generating precise in vivo arterial endothelial hemodynamic microenvironments using microfluidics is essential for exploring endothelial mechanobiology. However, a hemodynamic principle guiding the fabrication of microfluidic systems is still lacking. We propose a hemodynamic similarity principle for quickly obtaining the input impedance of the microfluidic system in vitro derived from that of the arterial system in vivo to precisely generate the desired endothelial hemodynamic microenvironments. First, based on the equivalent of blood pressure (BP) and wall shear stress (WSS) waveforms, we establish a hemodynamic similarity principle to efficiently map the input impedance in vivo to that in vitro, after which the multi-component microfluidic system is designed and fabricated using a lumped parameter hemodynamic model. Second, numerical simulation and experimental studies are carried out to validate the performance of the designed microfluidic system. Finally, the intracellular Ca2+ responses after exposure to different intensities of exercise-induced BP and WSS waveforms are measured to improve the reliability of EC mechanobiological studies using the designed microfluidic system. Overall, the proposed hemodynamic similarity principle can guide the fabrication of a multi-component microfluidic system for endothelial cell mechanobiology.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Endoteliais / Microfluídica Tipo de estudo: Prognostic_studies Idioma: En Revista: Talanta Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Endoteliais / Microfluídica Tipo de estudo: Prognostic_studies Idioma: En Revista: Talanta Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China