Electrochemical fabrication of Co(OH)2 nanoparticles decorated carbon cloth for non-enzymatic glucose and uric acid detection.

ABSTRACT

Cobalt hydroxide nanoparticles (Co(OH)2 NPs) were uniformly deposited on flexible carbon cloth substrate (Co(OH)2@CC) rapidly by a facile one-step electrodeposition, which can act as an enzyme-free glucose and uric acid sensor in an alkaline electrolyte. Compositional and morphological characterization were examined by X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDS), which confirmed the deposited nanospheres were Co(OH)2 nanoparticles (NPs). The electrochemical oxidation of glucose and uric acid at Co(OH)2@CC electrode was investigated by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), differential pulse voltammetry (DPV), and chronoamperometry methods. The results revealed a remarkable electrocatalytic activity toward the single and simultaneous determination of glucose and uric acid at about 0.6 V and 0.3 V (vs. Ag/AgCl), respectively, which is attributed to a noticeable synergy effect between Co(OH)2 NPs and CC with good repeatability, satisfactory reproducibility, considerable long-term stability, superior selectivity, outstanding sensitivity, and wide linear detection range from 1 uM to 2 mM and 25 nM to 1.5 uM for glucose and UA, respectively. The detection limits were 0.36 nM for UA and 0.24 µM for glucose (S/N = 3). Finally, the Co(OH)2@CC electrode was utilized for glucose and uric acid determination in human blood samples and satisfying results were obtained. The relative standard derivations (RSDs) for glucose and UA were in the range 6 to 14% and 0 to 3%, respectively. The recovery ranges for glucose an UA were 97 to 103% and 95 and 101%, respectively. These features make the novel Co(OH)2@CC sensor developed by a low-cost, efficient, and eco-friendly preparation method a potentially practical candidate for application to biosensors.