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Novel method for detecting complement C3 deposition on Staphylococcus aureus.
Wonfor, Toska; Li, Shuxian; Dunphy, Rhys W; Macpherson, Alex; van den Elsen, Jean; Laabei, Maisem.
Afiliação
  • Wonfor T; Department of Life Sciences, University of Bath, Bath, UK.
  • Li S; Department of Life Sciences, University of Bath, Bath, UK.
  • Dunphy RW; Department of Life Sciences, University of Bath, Bath, UK.
  • Macpherson A; Department of Life Sciences, University of Bath, Bath, UK.
  • van den Elsen J; UCB Biopharma UK, Slough, UK.
  • Laabei M; Department of Life Sciences, University of Bath, Bath, UK.
Sci Rep ; 12(1): 15766, 2022 09 21.
Article em En | MEDLINE | ID: mdl-36130996
The primary host response to Staphylococcus aureus infection occurs via complement. Complement is an elegant evolutionarily conserved system, playing essential roles in early defences by working in concert with immune cells to survey, label and destroy microbial intruders and coordinate inflammation. Currently the exact mechanisms employed by S. aureus to manipulate and evade complement is not clear and is hindered by the lack of accurate molecular tools that can report on complement deposition on the bacterial surface. Current gold-standard detection methods employ labelled complement-specific antibodies and flow cytometry to determine complement deposited on bacteria. These methods are restricted by virtue of the expression of the S. aureus immunoglobulin binding proteins, Protein A and Sbi. In this study we describe the use of a novel antibody-independent C3 probe derived from the staphylococcal Sbi protein, specifically Sbi-IV domain. Here we show that biotin-labelled Sbi-IV interacts specifically with deposited C3 products on the staphylococcal surface and thus can be used to measure complement fixation on wild-type cells expressing a full repertoire of immune evasion proteins. Lastly, our data indicates that genetically diverse S. aureus strains restrict complement to different degrees suggesting that complement evasion is a variable virulence trait among S. aureus isolates.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Estafilocócicas / Staphylococcus aureus Limite: Humans Idioma: En Revista: Sci Rep Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Estafilocócicas / Staphylococcus aureus Limite: Humans Idioma: En Revista: Sci Rep Ano de publicação: 2022 Tipo de documento: Article