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Development of a Molecular Marker Based on the Mitochondrial Genome for Detection of Cyclospora cayetanensis in Food and Water Samples.
Durigan, Mauricio; Patregnani, Emma; Gopinath, Gopal R; Ewing-Peeples, Laura; Lee, Chaeyoon; Murphy, Helen R; Almeria, Sonia; Cinar, Hediye Nese; Negrete, Flavia; da Silva, Alexandre J.
Afiliação
  • Durigan M; Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.
  • Patregnani E; Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.
  • Gopinath GR; Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.
  • Ewing-Peeples L; Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.
  • Lee C; Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.
  • Murphy HR; Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.
  • Almeria S; Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.
  • Cinar HN; Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.
  • Negrete F; Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.
  • da Silva AJ; Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.
Microorganisms ; 10(9)2022 Aug 31.
Article em En | MEDLINE | ID: mdl-36144364
ABSTRACT
Cyclospora cayetanensis is a coccidian parasite that causes diarrheal illness outbreaks worldwide. The development of new laboratory methods for detection of C. cayetanensis is of critical importance because of the high potential for environmental samples to be contaminated with a myriad of microorganisms, adversely impacting the specificity when testing samples from various sources using a single molecular assay. In this study, a new sequencing-based method was designed targeting a specific fragment of C. cayetanensis cytochrome oxidase gene and developed as a complementary method to the TaqMan qPCR present in the U.S. FDA BAM Chapter 19b and Chapter 19c. The comparative results between the new PCR protocol and the qPCR for detection of C. cayetanensis in food and water samples provided similar results in both matrices with the same seeding level. The target region and primers in the protocol discussed in this study contain sufficient Cyclospora-specific sequence fidelity as observed by sequence comparison with other Eimeriidae species. The sequence of the PCR product appears to represent a robust target for identifying C. cayetanensis on samples from different sources. Such a sensitive method for detection of C. cayetanensis would add to the target repertoire of qPCR-based screening strategies for food and water samples.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Idioma: En Revista: Microorganisms Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Idioma: En Revista: Microorganisms Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos