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Localization Patterns of RAB3C Are Associated with Murine and Human Sperm Formation.
Tsai, Yieh-Loong; Lai, Tsung-Hsuan; Liu, Hsuan-Che; Wang, Ya-Yun; Lin, Yu-Hua; Ke, Chih-Chun; Chung, Ming-Ting; Chan, Chying-Chyuan; Lin, Ying-Hung.
Afiliação
  • Tsai YL; Department of Obstetrics and Gynecology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei 111, Taiwan.
  • Lai TH; College of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan.
  • Liu HC; College of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan.
  • Wang YY; Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei 106, Taiwan.
  • Lin YH; Graduate Institute of Biomedical and Pharmaceutical Science, Fu Jen Catholic University, New Taipei City 242, Taiwan.
  • Ke CC; Graduate Institute of Biomedical and Pharmaceutical Science, Fu Jen Catholic University, New Taipei City 242, Taiwan.
  • Chung MT; Department of Chemistry, Fu Jen Catholic University, New Taipei City 242, Taiwan.
  • Chan CC; Division of Urology, Department of Surgery, Cardinal Tien Hospital, New Taipei City 231, Taiwan.
  • Lin YH; Ph.D Program in Nutrition and Food Science, Fu Jen Catholic University, New Taipei City 242, Taiwan.
Medicina (Kaunas) ; 58(10)2022 Oct 07.
Article em En | MEDLINE | ID: mdl-36295569
Background and Objectives: Septins (SEPTs) are highly conserved GTP-binding proteins and the fourth component of the cytoskeleton. Polymerization of SEPTs contributes to several critical cellular processes such as cytokinesis, cytoskeletal remodeling, and vesicle transportation. In our previous study, we found that SEPT14 mutations resulted in teratozoospermia with >87% sperm morphological defects. SEPT14 interactors were also identified through proteomic assays, and one of the peptides was mapped to RAB3B and RAB3C. Most studies on the RAB3 family have focused on RAB3A, which regulates the exocytosis of neurotransmitters and acrosome reactions. However, the general expression and patterns of the RAB3 family members during human spermatogenesis, and the association between RAB3 and teratozoospermia owing to a SEPT14 mutation, are largely unknown. Materials and Methods: Human sperm and murine male germ cells were collected in this study and immunofluorescence analysis was applied on the collected sperm. Results: In this study, we observed that the RAB3C transcripts were more abundant than those of RAB3A, 3B, and 3D in human testicular tissues. During human spermatogenesis, the RAB3C protein is mainly enriched in elongated spermatids, and RAB3B is undetectable. In mature human spermatozoa, RAB3C is concentrated in the postacrosomal region, neck, and midpiece. The RAB3C signals were delocalized within human spermatozoa harboring the SEPT14 mutation, and the decreased signals were accompanied by a defective head and tail, compared with the healthy controls. To determine whether RAB3C is involved in the morphological formation of the head and tail of the sperm, we separated murine testicular tissue and isolated elongated spermatids for further study. We found that RAB3C is particularly expressed in the manchette structure, which assists sperm head shaping at the spermatid head, and is also localized at the sperm tail. Conclusions: Based on these results, we suggest that the localization of RAB3C proteins in murine and human sperm is associated with SEPT14 mutation-induced morphological defects in sperm.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Teratozoospermia Tipo de estudo: Risk_factors_studies Limite: Animals / Humans / Male Idioma: En Revista: Medicina (Kaunas) Assunto da revista: MEDICINA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Taiwan

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Teratozoospermia Tipo de estudo: Risk_factors_studies Limite: Animals / Humans / Male Idioma: En Revista: Medicina (Kaunas) Assunto da revista: MEDICINA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Taiwan