New insights into the dynamics of causative dinoflagellates and the related contamination of molluscs by paralytic toxins in the southwestern Mediterranean coastal waters of Morocco.

ABSTRACT

The distribution of the two potentially toxic dinoflagellates Gymnodinium catenatum and Alexandrium spp. was investigated in the Mediterranean Moroccan Sea from March 2018 to March 2019. The cockle Acanthocardia tuberculata and the smooth clam Callista chione were collected at four stations, and their toxin levels were assessed using the mouse bioassay. The toxin profile was analysed by LC-MS/MS in G. catenatum and in the bivalves harvested in M'diq and Djawn. The species G. catenatum was present throughout the year, whereas Alexandrium spp. was less abundant. The paralytic shellfish toxin (PST) level in cockles was, on average, six times above the sanitary threshold; GTX5 was the major contributor to the total PST level, followed by dc-STX and STX. The toxin level of the smooth clam was considerably lower than that of the cockle; GTX5 and C-toxins were the dominating analogues. Our results suggest the responsibility of G. catenatum for the recurrent PST contamination in the Moroccan Mediterranean Sea, with a west-east gradient.