Development of molecular inversion probes for soybean progeny genomic selection genotyping.
Plant Genome
; 16(1): e20270, 2023 03.
Article
em En
| MEDLINE
| ID: mdl-36411593
ABSTRACT
Increasing rate of genetic gain for key agronomic traits through genomic selection requires the development of new molecular methods to run genome-wide single-nucleotide polymorphisms (SNPs). The main limitation of current methods is the cost is too high to screen breeding populations. Molecular inversion probes (MIPs) are a targeted genotyping-by-sequencing (GBS) method that could be used for soybean [Glycine max (L.) Merr.] that is both cost-effective, high-throughput, and provides high data quality to screen breeder's germplasm for genomic selection. A 1K MIP SNP set was developed for soybean with uniformly distributed markers across the genome. The SNPs were selected to maximize the number of informative markers in germplasm being tested in soybean breeding programs located in the northern-central and middle-southern regions of the United States. The 1K SNP MIP set was tested on diverse germplasm and a recombinant inbred line (RIL) population. Targeted sequencing with MIPs obtained an 85% enrichment for the targeted SNPs. The MIP genotyping accuracy was 93% overall, whereas homozygous call accuracy was 98% with <10% missing data. The accuracy of MIPs combined with its low per-sample cost makes it a powerful tool to enable genomic selection within soybean breeding programs.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Seleção Genética
/
Glycine max
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Sondas Moleculares
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Técnicas de Sonda Molecular
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Genoma de Planta
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Genômica
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Técnicas de Genotipagem
País/Região como assunto:
America do norte
Idioma:
En
Revista:
Plant Genome
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Estados Unidos