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Long non-coding RNA HOXC-AS1 exerts its oncogenic effects in esophageal squamous cell carcinoma by interaction with IGF2BP2 to stabilize SIRT1 expression.
Yang, Zhengwu; Wan, Junhu; Ma, Liwei; Li, Zhuofang; Yang, Ruotong; Yang, Haijun; Li, Junkuo; Zhou, Fuyou; Ming, Liang.
Afiliação
  • Yang Z; Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, and the Key Clinical Laboratory of Henan Province, Henan, China.
  • Wan J; Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, and the Key Clinical Laboratory of Henan Province, Henan, China.
  • Ma L; Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, and the Key Clinical Laboratory of Henan Province, Henan, China.
  • Li Z; Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, and the Key Clinical Laboratory of Henan Province, Henan, China.
  • Yang R; Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, and the Key Clinical Laboratory of Henan Province, Henan, China.
  • Yang H; Henan Key Medical Laboratory of Precise Prevention and Treatment of Esophageal Cancer, Anyang, China.
  • Li J; Department of Pathology, Anyang Cancer Hospital, The Forth Affiliated Hospital of Henan University of Science and Technology, Henan, China.
  • Zhou F; Thoracic Department, Anyang Tumor Hospital, Henan Key Medical Laboratory of Precise Prevention and Treatment of Esophageal Cancer, Anyang, China.
  • Ming L; Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, and the Key Clinical Laboratory of Henan Province, Henan, China.
J Clin Lab Anal ; 37(1): e24801, 2023 Jan.
Article em En | MEDLINE | ID: mdl-36510377
BACKGROUND: Long non-coding RNA HOXC cluster antisense RNA 1 (HOXC-AS1) is a novel lncRNA whose cancer-promoting effect in gastric cancer and nasopharyngeal carcinoma has already been demonstrated. However, its functions in esophageal squamous cell carcinoma (ESCC) remains unknown. LncRNAs can interact with RNA-binding proteins (RBPs) and affect gene expression levels through post-transcriptional regulation. Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) is a widely studied RBP, and sirtuin 1 also known as SIRT1 has been reported to be involved in cancer progression. METHODS: Establishment of in vivo models, HE and immunohistochemistry staining verified the oncogenic effect of HOXC-AS1. The interaction relationship between HOXC-AS1, IGF2BP2 and SIRT1 was verified by RNA pulldown and RNA immunoprecipitation (RIP) assay. Relative expression and stability changes of genes were detected by qPCR and actinomycin D experiments. Finally, the effect of HOXC-AS1-IGF2BP2-SIRT1 axis on ESCC was verified by rescue experiments. RESULTS: HOXC-AS1 is highly expressed in ESCC cells and plays oncogenic effects in vivo. qPCR showed the positive relationship between HOXC-AS1 and SIRT1 following HOXC-AS1 knockdown or overexpression. RNA-pulldown, mass spectrometry and RIP assay demonstrated that IGF2BP2 is an RBP downstream of HOXC-AS1. Then, RIP and qPCR showed that IGF2BP2 could bind to SIRT1 mRNA and knockdown IGF2BP2 resulted in decreased SIRT1 mRNA level. Finally, a series of rescue assay showed that the HOXC-AS1-IGF2BP2-SIRT1 axis can affect the function of ESCC. CONCLUSION: LncRNA HOXC-AS1 acts as an oncogenic role in ESCC, which impacts ESCC progression by interaction with IGF2BP2 to stabilize SIRT1 expression.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Esofágicas / MicroRNAs / RNA Longo não Codificante / Carcinoma de Células Escamosas do Esôfago Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Clin Lab Anal Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Esofágicas / MicroRNAs / RNA Longo não Codificante / Carcinoma de Células Escamosas do Esôfago Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Clin Lab Anal Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China