Single-cell RNA-seq of Lotus japonicus provide insights into identification and function of root cell types of legume.
J Integr Plant Biol
; 65(5): 1147-1152, 2023 May.
Article
em En
| MEDLINE
| ID: mdl-36537698
ABSTRACT
The roots of legume plant play a crucial role in nitrogen fixation. However, the transcriptomes of different cell types of legume root and their functions remain largely unknown. Here, we performed single-cell RNA sequencing and profiled more than 22,000 single cells from root tips of Lotus japonicus, a model species of legume. We identified seven clusters corresponding to seven major cell types, which were validated by in situ hybridization. Further analysis revealed regulatory programs including phytohormone and nodulation associated with specific cell types, and revealed conserved and diverged features for the cell types. Our results represent the first single-cell resolution transcriptome for legume root tips and a valuable resource for studying the developmental and physiological functions of various cell types in legumes.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lotus
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Integr Plant Biol
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
China