Your browser doesn't support javascript.
loading
Partial collapse of DNA tetrahedron for miRNA assay with duplex-specific nuclease-assisted amplification.
Zhu, Jinwen; Guo, Zhenzhen; Cui, Jinjiang; Miao, Peng.
Afiliação
  • Zhu J; University of Science and Technology of China, Hefei 230026, China.
  • Guo Z; Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China. cuijj@sibet.ac.cn.
  • Cui J; Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China. cuijj@sibet.ac.cn.
  • Miao P; University of Science and Technology of China, Hefei 230026, China.
Analyst ; 148(3): 512-515, 2023 Jan 31.
Article em En | MEDLINE | ID: mdl-36648312
We establish a facile electrochemical approach for detecting miRNA. Programmable DNA tetrahedron is designed using thiol groups for electrode modification, the amino group for the localization of electrochemical species and a hairpin structure that responds to target miRNA. In addition, duplex-specific nuclease-assisted amplification helps improve the sensitivity of this biosensor. The target-initiated partial collapse of the DNA tetrahedron event integrates recognition, electrode immobilization, signal recruitment and amplification. By measuring the sharp silver stripping peak, the highly sensitive detection of miRNA is achieved, which also performs satisfactorily challenging biological samples. This method is featured with simple operation, high sensitivity and practical utility, exhibiting great application potential in clinical diagnosis.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / MicroRNAs Idioma: En Revista: Analyst Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / MicroRNAs Idioma: En Revista: Analyst Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China