Your browser doesn't support javascript.
loading
Supplementation of GelMA with Minimally Processed Tissue Promotes the Formation of Densely Packed Skeletal-Muscle-Like Tissues.
Tavares-Negrete, Jorge A; Pedroza-González, Sara Cristina; Frías-Sánchez, Ada I; Salas-Ramírez, Miriam L; de Santiago-Miramontes, María de Los Ángeles; Luna-Aguirre, Claudia Maribel; Alvarez, Mario M; Trujillo-de Santiago, Grissel.
Afiliação
  • Tavares-Negrete JA; Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, 64849 Monterrey, México.
  • Pedroza-González SC; Departamento de Ingeniería Mecatrónica y Eléctrica, Tecnológico de Monterrey, 64849 Monterrey, México.
  • Frías-Sánchez AI; Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, 64849 Monterrey, México.
  • Salas-Ramírez ML; Departamento de Ingeniería Mecatrónica y Eléctrica, Tecnológico de Monterrey, 64849 Monterrey, México.
  • de Santiago-Miramontes MLÁ; Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, 64849 Monterrey, México.
  • Luna-Aguirre CM; Departamento de Ingeniería Mecatrónica y Eléctrica, Tecnológico de Monterrey, 64849 Monterrey, México.
  • Alvarez MM; Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, 64849 Monterrey, México.
  • Trujillo-de Santiago G; Departamento de Ingeniería Mecatrónica y Eléctrica, Tecnológico de Monterrey, 64849 Monterrey, México.
ACS Biomater Sci Eng ; 9(6): 3462-3475, 2023 06 12.
Article em En | MEDLINE | ID: mdl-37126642
ABSTRACT
We present a simple and cost-effective strategy for developing gelatin methacryloyl (GelMA) hydrogels supplemented with minimally processed tissue (MPT) to fabricate densely packed skeletal-muscle-like tissues. MPT powder was prepared from skeletal muscle by freeze-drying, grinding, and sieving. Cell-culture experiments showed that the incorporation of 0.5-2.0% (w/v) MPT into GelMA hydrogels enhances the proliferation of murine myoblasts (C2C12 cells) compared to proliferation in pristine GelMA hydrogels and GelMA supplemented with decellularized skeletal-muscle tissues (DCTs). MPT-supplemented constructs also preserved their three-dimensional (3D) integrity for 28 days. By contrast, analogous pristine GelMA constructs only maintained their structure for 14 days or less. C2C12 cells embedded in MPT-supplemented constructs exhibited a higher degree of cell alignment and reached a significantly higher density than cells loaded in pristine GelMA constructs. Our results suggest that the addition of MPT incorporates a rich source of biochemical and topological cues, such as growth factors, glycosaminoglycans (GAGs), and structurally preserved proteins (e.g., collagen). In addition, GelMA supplemented with MPT showed suitable rheological properties for use as bioinks for extrusion bioprinting. We envision that this simple and cost-effective strategy of hydrogel supplementation will evolve into an exciting spectrum of applications for tissue engineers, primarily in the biofabrication of relevant microtissues for in vitro models and cultured meat and ultimately for the biofabrication of transplant materials using autologous MPT.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Alicerces Teciduais / Impressão Tridimensional Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: ACS Biomater Sci Eng Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Alicerces Teciduais / Impressão Tridimensional Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: ACS Biomater Sci Eng Ano de publicação: 2023 Tipo de documento: Article