Inhibitory Effect of Pyra-Metho-Carnil on Cancer Spheroid Growth Through Decrease in Glycolysis-associated Molecules.
Anticancer Res
; 43(8): 3717-3726, 2023 Aug.
Article
em En
| MEDLINE
| ID: mdl-37500165
ABSTRACT
BACKGROUND/AIM:
Pyra-Metho-Carnil (PMC) has been identified as a novel candidate compound for treating numerous malignancies; however, its mechanism of action remains unknown. In this study, we conducted RNA-sequencing (RNA-seq) analyses to elucidate the mechanism of PMC against human colorectal cancer cells harboring mutant KRAS (mtKRAS). MATERIALS ANDMETHODS:
RNA-seq analyses of the HKe3-wild-type KRAS and HKe3-mtKRAS spheroids treated with DMSO or PMC for 6 days were performed.RESULTS:
RNA-seq data suggested that PMC treatment suppresses the aerobic glycolysis pathway in HKe3-mtKRAS spheroids through the down-regulation of the HIF1 pathway. Indeed, treatment with PMC markedly suppresses the absorption of glucose by spheroids and the secretion of lactate from them.CONCLUSION:
PMC suppresses growth of cancer spheroid through down-regulation of cancer-specific glucose metabolism.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Colorretais
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Anticancer Res
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Japão