Topological capture of mRNA for silencing gene expression.

Lyu, Fangjie; Tomita, Takashi; Abe, Naoko; Hiraoka, Haruka; Hashiya, Fumitaka; Nakashima, Yuko; Kajihara, Shiryu; Tomoike, Fumiaki; Shu, Zhaoma; Onizuka, Kazumitsu; Kimura, Yasuaki; Abe, Hiroshi

Lyu, Fangjie; Tomita, Takashi; Abe, Naoko; Hiraoka, Haruka; Hashiya, Fumitaka; Nakashima, Yuko; Kajihara, Shiryu; Tomoike, Fumiaki; Shu, Zhaoma; Onizuka, Kazumitsu; Kimura, Yasuaki; Abe, Hiroshi.

Afiliação

Lyu F; Department of Chemistry, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan. h-abe@chem.nagoya-u.ac.jp.
Tomita T; Department of Chemistry, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan. h-abe@chem.nagoya-u.ac.jp.
Abe N; Department of Chemistry, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan. h-abe@chem.nagoya-u.ac.jp.
Hiraoka H; Department of Chemistry, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan. h-abe@chem.nagoya-u.ac.jp.
Hashiya F; Department of Chemistry, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan. h-abe@chem.nagoya-u.ac.jp.
Nakashima Y; Department of Chemistry, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan. h-abe@chem.nagoya-u.ac.jp.
Kajihara S; Department of Chemistry, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan. h-abe@chem.nagoya-u.ac.jp.
Tomoike F; Research Center for Materials Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan.
Shu Z; Department of Chemistry, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan. h-abe@chem.nagoya-u.ac.jp.
Onizuka K; Institute of Multidisciplinary Research for Advanced Materials, Tohoku University 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi 980-8577, Japan.
Kimura Y; Department of Chemistry, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan. h-abe@chem.nagoya-u.ac.jp.
Abe H; Department of Chemistry, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan. h-abe@chem.nagoya-u.ac.jp.

Chem Commun (Camb) ; 59(77): 11564-11567, 2023 Sep 26.

Article em En | MEDLINE | ID: mdl-37682012

ABSTRACT

ABSTRACT

We describe herein topological mRNA capture using branched oligodeoxynucleotides (ODNs) with multiple reactive functional groups. These fragmented ODNs efficiently formed topological complexes on template mRNA in vitro. In cell-based experiments targeting AcGFP mRNA, the bifurcated reactive ODNs showed a much larger gene silencing effect than the corresponding natural antisense ODN.

Assuntos

Inativação Gênica; Oligodesoxirribonucleotídeos; RNA Mensageiro/genética; Expressão Gênica

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligodesoxirribonucleotídeos / Inativação Gênica Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Japão

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