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The proteomic landscape of synaptic diversity across brain regions and cell types.
van Oostrum, Marc; Blok, Thomas M; Giandomenico, Stefano L; Tom Dieck, Susanne; Tushev, Georgi; Fürst, Nicole; Langer, Julian D; Schuman, Erin M.
Afiliação
  • van Oostrum M; Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
  • Blok TM; Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
  • Giandomenico SL; Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
  • Tom Dieck S; Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
  • Tushev G; Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
  • Fürst N; Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
  • Langer JD; Max Planck Institute for Brain Research, Frankfurt am Main, Germany; Max Planck Institute of Biophysics, Frankfurt am Main, Germany.
  • Schuman EM; Max Planck Institute for Brain Research, Frankfurt am Main, Germany. Electronic address: erin.schuman@brain.mpg.de.
Cell ; 186(24): 5411-5427.e23, 2023 11 22.
Article em En | MEDLINE | ID: mdl-37918396
Neurons build synaptic contacts using different protein combinations that define the specificity, function, and plasticity potential of synapses; however, the diversity of synaptic proteomes remains largely unexplored. We prepared synaptosomes from 7 different transgenic mouse lines with fluorescently labeled presynaptic terminals. Combining microdissection of 5 different brain regions with fluorescent-activated synaptosome sorting (FASS), we isolated and analyzed the proteomes of 18 different synapse types. We discovered ∼1,800 unique synapse-type-enriched proteins and allocated thousands of proteins to different types of synapses (https://syndive.org/). We identify shared synaptic protein modules and highlight the proteomic hotspots for synapse specialization. We reveal unique and common features of the striatal dopaminergic proteome and discover the proteome signatures that relate to the functional properties of different interneuron classes. This study provides a molecular systems-biology analysis of synapses and a framework to integrate proteomic information for synapse subtypes of interest with cellular or circuit-level experiments.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sinapses / Encéfalo / Proteoma Limite: Animals Idioma: En Revista: Cell Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sinapses / Encéfalo / Proteoma Limite: Animals Idioma: En Revista: Cell Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Alemanha