Your browser doesn't support javascript.
loading
Multiomic Droplet-Based Assay for Ultralow Input Samples.
Richerd, Mathilde; Dumas, Simon; Hajji, Ismail; Serra, Marco; Descroix, Stéphanie.
Afiliação
  • Richerd M; Institut Curie, Laboratoire PhysicoChimie (CNRS UMR 168), Institut Pierre-Gilles de Gennes, Sorbonne Université, PSL Research University, 6 rue Jean, Calvin 75005, Paris, France.
  • Dumas S; Institut Curie, Laboratoire PhysicoChimie (CNRS UMR 168), Institut Pierre-Gilles de Gennes, Sorbonne Université, PSL Research University, 6 rue Jean, Calvin 75005, Paris, France.
  • Hajji I; Institut Curie, Laboratoire PhysicoChimie (CNRS UMR 168), Institut Pierre-Gilles de Gennes, Sorbonne Université, PSL Research University, 6 rue Jean, Calvin 75005, Paris, France.
  • Serra M; Institut Curie, Laboratoire PhysicoChimie (CNRS UMR 168), Institut Pierre-Gilles de Gennes, Sorbonne Université, PSL Research University, 6 rue Jean, Calvin 75005, Paris, France.
  • Descroix S; Institut Curie, Laboratoire PhysicoChimie (CNRS UMR 168), Institut Pierre-Gilles de Gennes, Sorbonne Université, PSL Research University, 6 rue Jean, Calvin 75005, Paris, France.
Anal Chem ; 95(49): 17988-17996, 2023 12 12.
Article em En | MEDLINE | ID: mdl-38032406
The extraction and separation of cellular compounds are crucial steps in numerous biological protocols, particularly in multiomics studies, where several cellular modalities are examined simultaneously. While magnetic particle extraction is commonly used, it may not be applicable for ultralow input samples. Microfluidics has made possible the analysis of rare or low-materiality samples such as circulating tumor cells or single cells through miniaturization of numerous protocols. In this study, a microfluidics workflow for separating different cellular modalities from ultralow input samples is presented. This approach is based on magnetic tweezers technology, allowing the extraction and resuspension of magnetic particles between consecutive nanoliter droplets to perform multistep assays on small volumes. The ability to separate and recover mRNA and gDNA in samples containing less than 10 cells is demonstrated, achieving separation efficiency comparable to the one obtained with conventional pipetting but with a significantly lower amount of starting material, typically 1-2 orders of magnitude less.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Analíticas Microfluídicas Idioma: En Revista: Anal Chem Ano de publicação: 2023 Tipo de documento: Article País de afiliação: França

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Analíticas Microfluídicas Idioma: En Revista: Anal Chem Ano de publicação: 2023 Tipo de documento: Article País de afiliação: França