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Alteration of γδ T cell subsets in non-human primates transplanted with GGTA1 gene-deficient porcine blood vessels.
Lee, Sujin; Chung, Yun Shin; Lee, Kyo Won; Choi, Miran; Sonn, Chung Hee; Oh, Won Jun; Hong, Hun Gi; Shim, Joohyun; Choi, Kimyung; Kim, Sung Joo; Park, Jae Berm; Kim, Tae Jin.
Afiliação
  • Lee S; Department of Immunology, Graduate School of Basic Medical Science, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.
  • Chung YS; Department of Immunology, Graduate School of Basic Medical Science, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.
  • Lee KW; Center for Antimicrobial Resistance and Microbial Genetics, University of Ulsan College of Medicine, Seoul, Republic of Korea.
  • Choi M; Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
  • Sonn CH; Transplantation Research Center, Samsung Medical Center, Seoul, Republic of Korea.
  • Oh WJ; Transplantation Research Center, Samsung Medical Center, Seoul, Republic of Korea.
  • Hong HG; Department of Immunology, Graduate School of Basic Medical Science, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.
  • Shim J; Department of Immunology, Graduate School of Basic Medical Science, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.
  • Choi K; Department of Transgenic Animal Research, Optipharm, Inc., Cheongju, Chungcheongbuk-do, Republic of Korea.
  • Kim SJ; Department of Transgenic Animal Research, Optipharm, Inc., Cheongju, Chungcheongbuk-do, Republic of Korea.
  • Park JB; GenNBio Co., Ltd, Pyeongtaek, Gyeonggi-do, Republic of Korea.
  • Kim TJ; Center for Antimicrobial Resistance and Microbial Genetics, University of Ulsan College of Medicine, Seoul, Republic of Korea.
Xenotransplantation ; 31(1): e12838, 2024.
Article em En | MEDLINE | ID: mdl-38112053
ABSTRACT

BACKGROUND:

αGal-deficient xenografts are protected from hyperacute rejection during xenotransplantation but are still rejected more rapidly than allografts. Despite studies showing the roles of non-Gal antibodies and αß T cells in xenograft rejection, the involvement of γδ T cells in xenograft rejection has been limitedly investigated.

METHODS:

Six male cynomolgus monkeys were transplanted with porcine vessel xenografts from wild-type (n = 3) or GGTA1 knockout (n = 3) pigs. We measured the proportions and T cell receptor (TCR) repertoires of blood γδ T cells before and after xenotransplant. Grafted porcine vessel-infiltrating immune cells were visualized at the end of experiments.

RESULTS:

Blood γδ T cells expanded and infiltrated into the graft vessel adventitia following xenotransplantation of α-Gal-deficient pig blood vessels. Pre- and post-transplant analysis of γδ TCR repertoire revealed a transition in δ chain usage post-transplantation, with the expansion of several clonotypes of δ1, δ3, or δ7 chains. Furthermore, the distinctions between pre- and post-transplant δ chain usages were more prominent than those observed for γ chain usages.

CONCLUSION:

γδ TCR repertoire was significantly altered by xenotransplantation, suggesting the role of γδ T cells in sustained xenoreactive immune responses.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Primatas / Subpopulações de Linfócitos T Limite: Animals Idioma: En Revista: Xenotransplantation Assunto da revista: TRANSPLANTE Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Primatas / Subpopulações de Linfócitos T Limite: Animals Idioma: En Revista: Xenotransplantation Assunto da revista: TRANSPLANTE Ano de publicação: 2024 Tipo de documento: Article