Assessment of measurement accuracy of amplified DNA using a colorimetric loop-mediated isothermal amplification assay.
Biotechniques
; 76(3): 114-118, 2024 Mar.
Article
em En
| MEDLINE
| ID: mdl-38131320
ABSTRACT
A colorimetric loop-mediated isothermal amplification assay detects changes in pH during amplification based on color changes at a constant temperature. Currently, various studies have focused on developing and assessing molecular point-of-care testing instruments. In this study, we evaluated amplified DNA concentrations measured using the colorimetric LAMP assay of the 1POT™ Professional device (1drop Inc, Korea). Results of the 1POT analysis of clinical samples were compared with measurements obtained from the Qubit™ 4 and NanoDrop™ 2000 devices (both from Thermo Fisher Scientific, MA, USA). These results showed a correlation of 0.98 (95% CI 0.96-0.99) and 0.96 (95% CI 0.92-0.98) between 1POT and the Qubit and NanoDrop. 1POT can measure amplified DNA accurately and is suitable for on-site molecular diagnostics.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Colorimetria
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
2024
Tipo de documento:
Article