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Paenibacillus silvisoli sp. nov. and Paenibacillus humicola sp. nov., isolated from forest soil.
Lee, Hyosun; Chaudhary, Dhiraj Kumar; Lim, Oung Bin; Kim, Dong-Uk.
Afiliação
  • Lee H; Department of Biological Science, College of Science and Engineering, Sangji University, Wonju, 26339, Republic of Korea.
  • Chaudhary DK; Department of Environmental Engineering, College of Science and Technology, Korea University Sejong Campus, 2511 Sejong-ro, Sejong City, 30019, Republic of Korea.
  • Lim OB; Department of Biological Science, College of Science and Engineering, Sangji University, Wonju, 26339, Republic of Korea.
  • Kim DU; Department of Biological Science, College of Science and Engineering, Sangji University, Wonju, 26339, Republic of Korea. dukim@sangji.ac.kr.
Arch Microbiol ; 206(1): 42, 2023 Dec 26.
Article em En | MEDLINE | ID: mdl-38147140
ABSTRACT
During the study of microbial ecology of forest soil, two circular, white-colored bacterial colonies were isolated and labeled as strains TW38T and TW40T. Both strains were catalase positive and oxidase negative. Strains TW38T and TW40T demonstrated growth within a temperature range of 10-37 °C and 18-37 °C, respectively, and thrived within a pH range of 5.5-9.0 and 6.0-8.0, respectively. Both strains grew at 0-2.0% (w/v) NaCl concentrations. The phylogenetic analysis indicated that strains TW38T and TW40T affiliated to the genus Paenibacillus, with the closest neighbors being Paenibacillus montanisoli RA17T (98.6%) and Paenibacillus arachidis E3T (95.4%), respectively. In both strains, the sole respiratory quinone was MK-7, the signature fatty acid was antiso-C150, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine. The digital DNA-DNA hybridization and the average nucleotide identity values between TW38T, TW40T, and closest reference strains were < 29.0% and < 85.0%, respectively. The DNA G+C content of TW38T and TW40T was 54.5% and 57.1%, respectively. In general, the phylogenetic, genomics, chemotaxonomic, and phenotypic data support the differentiation of TW38T and TW40T from other closest members of the genus Paenibacillus. Thus, we conclude both strains TW38T and TW40T represent novel species of the genus Paenibacillus, for which the name Paenibacillus silvisoli sp. nov. and Paenibacillus humicola sp. nov. are proposed, respectively. The type strain of Paenibacillus silvisoli is TW38T (= KCTC 43468T = NBRC 116015T) and type strain of Paenibacillus humicola is TW40T (= KCTC 43469T = NBRC 116016T).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cardiolipinas / Paenibacillus Idioma: En Revista: Arch Microbiol Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cardiolipinas / Paenibacillus Idioma: En Revista: Arch Microbiol Ano de publicação: 2023 Tipo de documento: Article