Your browser doesn't support javascript.
loading
Molecular and clinicopathological features of KIT/PDGFRA wild-type gastrointestinal stromal tumors.
Nishida, Toshirou; Naito, Yoichi; Takahashi, Tsuyoshi; Saito, Takuro; Hisamori, Shigeo; Manaka, Dai; Ogawa, Katsuhiro; Hirota, Seiichi; Ichikawa, Hitoshi.
Afiliação
  • Nishida T; Department of Surgery, Japan Community Health-care Organization Osaka Hospital, Osaka, Japan.
  • Naito Y; Department of Surgery, National Cancer Center Hospital, Tokyo, Japan.
  • Takahashi T; National Institute of Biomedical Innovation, Health and Nutrition, Laboratory of Nuclear Transport Dynamics, Ibaraki, Japan.
  • Saito T; Department of General Internal Medicine, National Cancer Center Hospital East, Kashiwa, Japan.
  • Hisamori S; Department of Experimental Therapeutics, National Cancer Center Hospital East, Kashiwa, Japan.
  • Manaka D; Department of Medical Oncology, National Cancer Center Hospital East, Kashiwa, Japan.
  • Ogawa K; Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Suita, Japan.
  • Hirota S; Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Suita, Japan.
  • Ichikawa H; Department of Surgery, Osaka Police Hospital, Osaka, Japan.
Cancer Sci ; 115(3): 894-904, 2024 Mar.
Article em En | MEDLINE | ID: mdl-38178783
ABSTRACT
Approximately 10% of gastrointestinal stromal tumors (GISTs) harbor reportedly no KIT and PDGFRA mutations (wild-type GISTs). The clinicopathological features and oncologic outcomes of wild-type GISTs based on molecular profiles are unknown. We recruited 35 wild-type GIST patients from the two registry studies of high-risk GISTs between 2012 and 2015 and primary GISTs between 2003 and 2014. Molecular profiling of wild-type GISTs was performed by targeted next-generation sequencing (NGS) using formalin-fixed paraffin-embedded tumor samples. Among 35 wild-type GISTs, targeted NGS analysis detected NF1, SDH, or BRAF mutation 16 NF1-GISTs with various NF1 mutations, 12 SDH-GISTs (4 with SDHA mutations, 4 with SDHB mutations, and 4 with SDHB-negative staining), and 5 BRAF-GISTs with the V600E mutation. Two GISTs showed no mutations based on our targeted NGS analysis. Additional gene mutations were infrequent in primary wild-type GISTs and found in TP53, CREBBP, CDKN2A, and CHEK2. Most NF1-GISTs were located in the small intestine (N = 12; 75%) and showed spindle cell features (N = 15; 94%) and multiple tumors (N = 6, 38%) with modest proliferation activities. In contrast, SDH-GISTs were predominantly found in the stomach (N = 11; 92%), exhibiting epithelioid cell (N = 6; 50%) and multiple (N = 6, 50%) features. The overall survival of patients with SDH-GISTs appeared to be better than that of BRAF-GISTs (p = 0.0107) or NF1-GISTs (p = 0.0754), respectively. In conclusion, major molecular changes in wild-type GISTs include NF1, SDH, and BRAF. NF1-GISTs involved multifocal spindle cell tumors in the small intestine. SDH-GISTs occurred in young patients and were multifocal in the stomach and clinically indolent.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tumores do Estroma Gastrointestinal Limite: Humans Idioma: En Revista: Cancer Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Japão

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tumores do Estroma Gastrointestinal Limite: Humans Idioma: En Revista: Cancer Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Japão