Construction and characterization of a full-length infectious clone of an emerging senecavirus A strain.
Arch Virol
; 169(2): 25, 2024 Jan 12.
Article
em En
| MEDLINE
| ID: mdl-38214826
ABSTRACT
Senecavirus A (SVA) is an emerging virus that causes vesicular disease in pigs. Construction of a full-length SVA cDNA clone is crucial for understanding its replication and pathogenesis. Here, we successfully constructed a CMV-promoter-driven infectious cDNA clone of the SVA isolate SVA/GX/CH/2018, which we named rSVA GX01. Sequence comparison between the pSVA GX01 and the parental isolate (SVA/GX/CH/2018) revealed three single-nucleotide differences. Four-week-old piglets were experimentally infected with either the parental virus or the cloned virus. The results showed that the cloned rSVA GX01 displayed weak pathogenicity in 4-week-old pigs compared to the parental virus SVA CH-GX-01-2018. The infectious clone of SVA will serve as a valuable tool for studying the viral replication cycle and for functional analysis of the viral genome.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Picornaviridae
/
Doenças dos Suínos
/
Infecções por Picornaviridae
Limite:
Animals
Idioma:
En
Revista:
Arch Virol
/
Arch. virol
/
Archives of virology
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China