Assessment of the efficiency of DNA isolation and profiling applying a temperature-driven method in human remains.
Electrophoresis
; 45(9-10): 805-813, 2024 May.
Article
em En
| MEDLINE
| ID: mdl-38247192
ABSTRACT
The identification of human remains is of utmost importance in a variety of scenarios. One of the primary identification methods is DNA. DNA extraction from human remains could be difficult, particularly in situations where the remains have been exposed to environmental conditions and other insults. Several studies tried to improve extraction by applying different approaches. ForensicGEM Universal (MicroGem) is a single-tube approach to DNA extraction and a temperature-driven method that could have some advantages with respect to previous techniques, among them, reducing the risk of contamination, not requiring specialized equipment, or several steps to perform. The aim of this study was to assess, for the first time, the efficiency of DNA extraction and quality of STR profiles applying the MicroGem protocol and modifications of this protocol from tooth samples in comparison with automatic extraction (AE). Our results indicated that AE and MicroGem performed similar, though with variability depending on the MicroGem modifications, increasing the DNA yield and STR profile quality when DNA is concentrated with Microcon. These findings demonstrated the efficiency of this methodology for DNA extraction from human remains while also providing a simple and quick technique suitable to apply in a variety of forensic scenarios.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Temperatura
/
DNA
/
Impressões Digitais de DNA
/
Repetições de Microssatélites
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Electrophoresis
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos