Production of Influenza Virus Glycoproteins Using Insect Cells.

Loganathan, Madhumathi; Francis, Benjamin; Krammer, Florian

Loganathan, Madhumathi; Francis, Benjamin; Krammer, Florian.

Afiliação

Loganathan M; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Francis B; Center for Vaccine Research and Pandemic Preparedness (C-VaRPP), Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Krammer F; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

Methods Mol Biol ; 2762: 43-70, 2024.

Article em En | MEDLINE | ID: mdl-38315359

ABSTRACT

ABSTRACT

The baculovirus/insect cell expression system is a very useful tool for reagent and antigen generation in vaccinology, virology, and immunology. It allows for the production of recombinant glycoproteins, which are used as antigens in vaccination studies and as reagents in immunological assays. Here, we describe the process of recombinant glycoprotein production using the baculovirus/insect cell expression system.

Assuntos

Virus da Influenza A Subtipo H5N1; Vacinas contra Influenza; Animais; Baculoviridae/genética; Baculoviridae/metabolismo; Glicoproteínas/genética; Glicoproteínas/metabolismo; Glicoproteínas de Hemaglutininação de Vírus da Influenza; Proteínas Recombinantes; Insetos/metabolismo

Palavras-chave

Baculovirus; Hemagglutinin; Influenza virus; Insect cells; Neuraminidase; Protein production; Viral glycoproteins

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vacinas contra Influenza / Virus da Influenza A Subtipo H5N1 Limite: Animals Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos

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