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Genome Identification and Expression Profiling of the PIN-Formed Gene Family in Phoebe bournei under Abiotic Stresses.
Li, Jingshu; Zhang, Yanzi; Tang, Xinghao; Liao, Wenhai; Li, Zhuoqun; Zheng, Qiumian; Wang, Yanhui; Chen, Shipin; Zheng, Ping; Cao, Shijiang.
Afiliação
  • Li J; College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
  • Zhang Y; University Key Laboratory of Forest Stress Physiology, Ecology and Molecular Biology of Fujian Province, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
  • Tang X; FAFU-UCR Joint Center for Horticultural Plant Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
  • Liao W; College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
  • Li Z; Fujian Academy of Forestry Sciences, Fuzhou 350012, China.
  • Zheng Q; College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
  • Wang Y; University Key Laboratory of Forest Stress Physiology, Ecology and Molecular Biology of Fujian Province, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
  • Chen S; College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
  • Zheng P; University Key Laboratory of Forest Stress Physiology, Ecology and Molecular Biology of Fujian Province, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
  • Cao S; College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Int J Mol Sci ; 25(3)2024 Jan 25.
Article em En | MEDLINE | ID: mdl-38338732
ABSTRACT
PIN-formed (PIN) proteins-specific transcription factors that are widely distributed in plants-play a pivotal role in regulating polar auxin transport, thus influencing plant growth, development, and abiotic stress responses. Although the identification and functional validation of PIN genes have been extensively explored in various plant species, their understanding in woody plants-particularly the endangered species Phoebe bournei (Hemsl.) Yang-remains limited. P. bournei is an economically significant tree species that is endemic to southern China. For this study, we employed bioinformatics approaches to screen and identify 13 members of the PIN gene family in P. bournei. Through a phylogenetic analysis, we classified these genes into five sub-families A, B, C, D, and E. Furthermore, we conducted a comprehensive analysis of the physicochemical properties, three-dimensional structures, conserved motifs, and gene structures of the PbPIN proteins. Our results demonstrate that all PbPIN genes consist of exons and introns, albeit with variations in their number and length, highlighting the conservation and evolutionary changes in PbPIN genes. The results of our collinearity analysis indicate that the expansion of the PbPIN gene family primarily occurred through segmental duplication. Additionally, by predicting cis-acting elements in their promoters, we inferred the potential involvement of PbPIN genes in plant hormone and abiotic stress responses. To investigate their expression patterns, we conducted a comprehensive expression profiling of PbPIN genes in different tissues. Notably, we observed differential expression levels of PbPINs across the various tissues. Moreover, we examined the expression profiles of five representative PbPIN genes under abiotic stress conditions, including heat, cold, salt, and drought stress. These experiments preliminarily verified their responsiveness and functional roles in mediating responses to abiotic stress. In summary, this study systematically analyzes the expression patterns of PIN genes and their response to abiotic stresses in P. bournei using whole-genome data. Our findings provide novel insights and valuable information for stress tolerance regulation in P. bournei. Moreover, the study offers significant contributions towards unraveling the functional characteristics of the PIN gene family.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Estresse Fisiológico Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Int J Mol Sci / Int. j. mol. sci. (Online) / International journal of molecular sciences (Online) Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Estresse Fisiológico Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Int J Mol Sci / Int. j. mol. sci. (Online) / International journal of molecular sciences (Online) Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China