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Unraveling biotypes of the northern house mosquito, Culex pipiens s.l. (Diptera: Culicidae): molecular differentiation and morphometric analysis.
Alvial, Ingrid E; Hernández-P, Raquel; Suazo, Manuel J; González, Christian R; Véliz, David; Benítez, Hugo A.
Afiliação
  • Alvial IE; Laboratorio de Ecología y Morfometría Evolutiva, Centro de Investigación de Estudios Avanzados del Maule, Universidad Católica del Maule, Talca, Chile.
  • Hernández-P R; Departamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad de México, México.
  • Suazo MJ; Instituto de Alta Investigación, Universidad de Tarapacá, Arica, Chile.
  • González CR; Instituto de Entomología, Facultad de Ciencias Básicas, Universidad Metropolitana de Ciencias de la Educación, Santiago, Chile.
  • Véliz D; Departamento de Ciencias Ecológicas, Universidad de Chile, Ñuñoa, Santiago, Chile.
  • Benítez HA; Centro de Ecología y Manejo de Islas Oceánicas (ESMOI), Coquimbo, Chile.
J Insect Sci ; 24(1)2024 Jan 01.
Article em En | MEDLINE | ID: mdl-38340048
ABSTRACT
Geometric morphometrics was used to determine whether geographic isolation could explain differences in wing size and shape between and within continental (27°S to 41°S) and insular (Rapa Nui) populations of Culex pipiens s.s. Linnaeus and their biotypes (f. pipiens and f. molestus). Molecular protocols based on polymorphisms in the second intron of nuclear locus ace-2 (acetylcholinesterase-2) were used to differentiate Cx. pipiens s.s. from Cx. quinquefasciatus Say, and an assay based on polymorphisms in the flanking region of a microsatellite locus (CQ11) was used to identify biotypes. Culex pipiens f. molestus and hybrids shared larval habitats in all continental sites, while Cx. pipiens f. pipiens was found in 5 of the 10 sites. Only biotype molestus was found in Rapa Nui (Easter Island) Pipiens and molestus biotypes occur sympatrically in aboveground locations, and only molestus was found in the underground site (ME). Biotype molestus was dominant in rural locations and preferably anthropophilic. These results agree with the ecological descriptions previously reported for the biotypes of Cx. pipiens s.s. Procrustes ANOVA only showed differences in centroid size between biotypes in females and males and did not show significant differences in wing shape. However, we found significant differences among the geographic areas in the centroid size and wing shape of both females and males. Particularly, the population of Rapa Nui Island had shorter wings than the continental populations. The results highlight the effects of geographic and environmental processes on morphotypes in vector mosquitoes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Culex / Culicidae Limite: Animals Idioma: En Revista: J Insect Sci Assunto da revista: BIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Chile

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Culex / Culicidae Limite: Animals Idioma: En Revista: J Insect Sci Assunto da revista: BIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Chile