Association of factor V activity with membranous vesicles released from human platelets: requirement for platelet stimulation.
Thromb Res
; 39(1): 49-61, 1985 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-3839943
ABSTRACT
The membrane-associated factor V-like activity (platelet factor 1, PF1) and the phospholipid-like catalytic surface activity (platelet factor 3, PF3) were studied in human platelets from normal and two factor V-deficient donors. Collagen stimulation or mechanical disruption of gel-filtered platelets was necessary for the expression of significant amounts of PF1 and PF3. Stimulation was also necessary for the uptake of factor V or Va by PF1-deficient platelets from the factor V-deficient donors. The activity of PF1 was also generated by association of factor V or Va with membrane-rich fractions obtained by gel filtration of the supernatant from collagen-stimulated or frozen-thawed PF1-deficient platelets. The amount of PF1 obtained by such all-or-none binding experiments was directly proportional to the amount of PF3 already expressed in the platelet preparation. These data have been summarized in terms of a hypothesis which views PF1 and PF3 to be activities associated with membranous vesicles released from platelets only after stimulation.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plaquetas
/
Fatores de Coagulação Sanguínea
/
Fator V
/
Fator Plaquetário 3
/
Deficiência do Fator V
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Thromb Res
Ano de publicação:
1985
Tipo de documento:
Article