3D printed microfluidic devices for integrated solid-phase extraction and microchip electrophoresis of preterm birth biomarkers.
Anal Chim Acta
; 1296: 342338, 2024 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-38401930
ABSTRACT
BACKGROUND:
Preterm birth (PTB) is a leading cause of neonatal mortality, such that the need for a rapid and accurate assessment for PTB risk is critical. Here, we developed a 3D printed microfluidic system that integrated solid-phase extraction (SPE) and microchip electrophoresis (µCE) of PTB biomarkers, enabling the combination of biomarker enrichment and labeling with µCE separation and fluorescence detection.RESULTS:
Reversed-phase SPE monoliths were photopolymerized in 3D printed devices. Microvalves in the device directed sample between the SPE monolith and the injection cross-channel in the serpentine µCE channel. Successful on-chip preconcentration, labeling and µCE separation of four PTB-related polypeptides were demonstrated in these integrated microfluidic devices. We further show the ability of these devices to handle complex sample matrices through the successful analysis of labeled PTB biomarkers spiked into maternal blood serum. The detection limit was 7 nM for the PTB biomarker, corticotropin releasing factor, in 3D printed SPE-µCE integrated devices.SIGNIFICANCE:
This work represents the first successful demonstration of integration of SPE and µCE separation of disease-linked biomarkers in 3D printed microfluidic devices. These studies open up promising possibilities for rapid bioanalysis of medically relevant analytes.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nascimento Prematuro
/
Eletroforese em Microchip
Limite:
Female
/
Humans
/
Newborn
Idioma:
En
Revista:
Anal Chim Acta
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos