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Variability of Isoquinoline Alkaloid Profiles and Anticholinesterase Activities with Binding-Mode Predictions of Glaucium flavum Population.
Bozkurt, Buket; Ulkar, Duygu; Nurlu, Necati; Coban, Gunes; Gumus, Zinar Pinar; Unver-Somer, Nehir.
Afiliação
  • Bozkurt B; Department of Pharmacognosy, Faculty of Pharmacy, Ege University, 35100, Bornova, Izmir, Türkiye.
  • Ulkar D; Department of Pharmacognosy, Faculty of Pharmacy, Ege University, 35100, Bornova, Izmir, Türkiye.
  • Nurlu N; Department of Pharmacognosy, Faculty of Pharmacy, Ege University, 35100, Bornova, Izmir, Türkiye.
  • Coban G; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ege University, 35100, Bornova, Izmir, Türkiye.
  • Gumus ZP; Ege University, Central Research Test and Analysis Laboratory Application and Research Center (EGE-MATAL), Ege University, 35100, Bornova, Izmir, Türkiye.
  • Unver-Somer N; Department of Pharmacognosy, Faculty of Pharmacy, Ege University, 35100, Bornova, Izmir, Türkiye.
Chem Biodivers ; 21(4): e202301865, 2024 Apr.
Article em En | MEDLINE | ID: mdl-38415909
ABSTRACT
In this study, phytochemical and biological activity studies supported by docking were carried out on a species of the genus Glaucium, a repository of isoquinoline alkaloids. The GC-MS (Gas Chromatography-Mass Spectrometry) method is used to characterize the isoquinoline alkaloids of Glaucium flavum Crantz. (Papaveraceae). G. flavum was collected from seven different regions of Türkiye (Antalya, Urla-Izmir, Mordogan-Izmir, Mugla, Assos-Canakkale, Karabiga-Canakkale, Giresun) and totally 17 compounds were detected by GC-MS. Glaucine was found to be the major constituent in the sample collected from Mugla, whereas isocorydine was recorded to be the principal alkaloid in other samples. Further fractionation studies on G. flavum collected from Antalya province in Southwestern Türkiye, yielded five major alkaloids (isocorydine 1, dihydrosanguinarine 2, glaucine 3, dehydroglaucine 4, protopine 5) which were characterized by spectroscopic methods. Anticholinesterase activities of the extracts and isolated alkaloids were also tested by in vitro Ellman method. The isolated compounds were also analyzed by a molecular docking technique to determine the binding orientations in the gorge of the active site of acetylcholinesterase (AChE) and a homology model of butyrylcholinesterase (BuChE). This is the first comparative investigation of the phytochemical composition and biodiversity of Glaucium flavum species growing in Türkiye.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Papaveraceae / Alcaloides / Antineoplásicos Idioma: En Revista: Chem Biodivers Assunto da revista: BIOQUIMICA / QUIMICA Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Papaveraceae / Alcaloides / Antineoplásicos Idioma: En Revista: Chem Biodivers Assunto da revista: BIOQUIMICA / QUIMICA Ano de publicação: 2024 Tipo de documento: Article