Stress response membrane protein OsSMP2 negatively regulates rice tolerance to drought.

Wang, Wei; Li, Yaqi; Yang, Songjin; Wu, Jiacheng; Ma, Chuan; Chen, Yulin; Sun, Xingzhuo; Wu, Lingli; Liang, Xin; Fu, Qiuping; Xu, Zhengjun; Li, Lihua; Huang, Zhengjian; Zhu, Jianqing; Jia, Xiaomei; Ye, Xiaoying; Chen, Rongjun

Wang, Wei; Li, Yaqi; Yang, Songjin; Wu, Jiacheng; Ma, Chuan; Chen, Yulin; Sun, Xingzhuo; Wu, Lingli; Liang, Xin; Fu, Qiuping; Xu, Zhengjun; Li, Lihua; Huang, Zhengjian; Zhu, Jianqing; Jia, Xiaomei; Ye, Xiaoying; Chen, Rongjun.

Afiliação

Wang W; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University of Rice Research Institute, Chengdu, 611130, China.
Li Y; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University of Rice Research Institute, Chengdu, 611130, China.
Yang S; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University of Rice Research Institute, Chengdu, 611130, China.
Wu J; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University of Rice Research Institute, Chengdu, 611130, China.
Ma C; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University of Rice Research Institute, Chengdu, 611130, China.
Chen Y; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University of Rice Research Institute, Chengdu, 611130, China.
Sun X; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University of Rice Research Institute, Chengdu, 611130, China.
Wu L; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University of Rice Research Institute, Chengdu, 611130, China.
Liang X; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University of Rice Research Institute, Chengdu, 611130, China.
Fu Q; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University of Rice Research Institute, Chengdu, 611130, China.
Xu Z; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University of Rice Research Institute, Chengdu, 611130, China.
Li L; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University of Rice Research Institute, Chengdu, 611130, China.
Huang Z; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University of Rice Research Institute, Chengdu, 611130, China.
Zhu J; Demonstration Base for International Science & Technology Cooperation of Sichuan Province, Sichuan Agricultural University 211, Huimin Road, Chengdu 611130, China.
Jia X; Demonstration Base for International Science & Technology Cooperation of Sichuan Province, Sichuan Agricultural University 211, Huimin Road, Chengdu 611130, China.
Ye X; Demonstration Base for International Science & Technology Cooperation of Sichuan Province, Sichuan Agricultural University 211, Huimin Road, Chengdu 611130, China.
Chen R; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University of Rice Research Institute, Chengdu, 611130, China.

J Exp Bot ; 75(11): 3300-3321, 2024 Jun 07.

Article em En | MEDLINE | ID: mdl-38447063

ABSTRACT

ABSTRACT

In a gene chip analysis, rice (Oryza sativa) OsSMP2 gene expression was induced under various abiotic stresses, prompting an investigation into its role in drought resistance and abscisic acid signaling. Subsequent experiments, including qRT-PCR and ß-glucuronidase activity detection, affirmed the OsSMP2 gene's predominant induction by drought stress. Subcellular localization experiments indicated the OsSMP2 protein primarily localizes to the cell membrane system. Overexpressing OsSMP2 increased sensitivity to exogenous abscisic acid, reducing drought resistance and leading to reactive oxygen species accumulation under drought stress. Conversely, in simulated drought experiments, OsSMP2-silenced transgenic plants showed significantly longer roots compared with the wild-type Nipponbare. These results suggest that OsSMP2 overexpression negatively affects rice drought resistance, offering valuable insights into molecular mechanisms, and highlight OsSMP2 as a potential target for enhancing crop resilience to drought stress.

Assuntos

Ácido Abscísico; Secas; Regulação da Expressão Gênica de Plantas; Oryza; Proteínas de Plantas; Estresse Fisiológico; Oryza/genética; Oryza/fisiologia; Oryza/metabolismo; Proteínas de Plantas/metabolismo; Proteínas de Plantas/genética; Ácido Abscísico/metabolismo; Plantas Geneticamente Modificadas; Proteínas de Membrana/metabolismo; Proteínas de Membrana/genética

Palavras-chave

Abscisic acid; drought; gene; reactive oxygen species; rice; roots

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Oryza / Estresse Fisiológico / Ácido Abscísico / Regulação da Expressão Gênica de Plantas / Secas Idioma: En Revista: J Exp Bot / J. exp. bot. (Online) / Journal of experimental botany (Online) Assunto da revista: BOTANICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

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