Evaluation of human papillomavirus type 16 viral load and genome physical status in Iranian women with cervical disease.
Mol Biol Rep
; 51(1): 411, 2024 Mar 11.
Article
em En
| MEDLINE
| ID: mdl-38466465
ABSTRACT
BACKGROUND:
This study examined the viral load and physical status of the human papillomavirus 16 (HPV-16) genome in non-cancerous, precancerous and cancerous cervical lesions.METHODS:
Quantitative real-time PCR was performed to determine HPV-16 E2 and E6 viral load in 132 cervical specimens. E2/E6 viral load ratio was used to determine the physical status of HPV-16 genome.RESULTS:
E2 gene viral load was a significant (P < 0.001) predicting biomarker in differentiating non-cancerous from precancerous and cancerous samples. E6 gene viral load was significantly different between the groups (P < 0.001). The specificity and sensitivity of E2 and E6 in distinguishing SCC samples were 100% and 95% respectively.CONCLUSION:
HPV-16 viral load measured through E2 and E6 genes is a reliable indicator of lesion type.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lesões Pré-Cancerosas
/
Neoplasias do Colo do Útero
/
Proteínas Oncogênicas Virais
/
Infecções por Papillomavirus
Limite:
Female
/
Humans
País/Região como assunto:
Asia
Idioma:
En
Revista:
Mol Biol Rep
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Irã