Effect of Vaginal Microecological Alterations on Female Pelvic Organ Prolapse.
Int Urogynecol J
; 35(4): 881-891, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38488886
ABSTRACT
INTRODUCTION AND HYPOTHESIS:
The objective was to investigate the correlation between endogenous vaginal microecological alterations and female pelvic organ prolapse (POP).METHODS:
Patients who underwent vaginal hysterectomy were retrospectively analyzed as the POP group (n = 30) and the non-POP group (n = 30). The vaginal microbial metabolites and enzyme levels were tested using the dry chemoenzymatic method. The mRNA and protein expression were tested using real-time quantitative PCR and immunohistochemistry. SPSS version 25.0 and GraphPad Prism 8.0 were performed for statistical analysis.RESULTS:
Compared with the non-POP group, the vaginal pH, H2O2 positivity and leukocyte esterase positivity were higher in patients with POP (all p < 0.05). Further analysis showed that patients with pelvic organ prolapse quantification (POP-Q) stage IV had higher rates of vaginal pH, H2O2 positivity and leukocyte esterase positivity than those with POP-Q stage III. Additionally, the mRNA expression of decorin (DCN), transforming growth factor beta 1 (TGF-ß1), and matrix metalloproteinase-3 (MMP-3) in uterosacral ligament tissues were higher, whereas collagen I and III were lower. Similarly, the positive expression of MMP-3 in uterosacral ligament tissue was significantly upregulated in the POP group compared with the non-POP group (p = 0.035), whereas collagen I (p = 0.004) and collagen III (p = 0.019) in uterosacral ligament tissue were significantly downregulated in the POP group. Correlation analysis revealed that there was a significant correlation between vaginal microecology and collagen metabolism. In addition, MMP-3 correlated negatively with collagen I and collagen III (p = 0.002, r = -0.533; p = 0.002, r = -0.534 respectively), whereas collagen I correlated positively with collagen III (p = 0.001, r = 0.578).CONCLUSIONS:
Vaginal microecological dysbiosis affects the occurrence of female POP, which could be considered a novel therapeutic option.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vagina
/
Prolapso de Órgão Pélvico
Limite:
Adult
/
Aged
/
Female
/
Humans
/
Middle aged
Idioma:
En
Revista:
Int Urogynecol J
/
Int. urogynecol. j. (Print)
/
International urogynecology journal (Print)
Assunto da revista:
GINECOLOGIA
/
UROLOGIA
Ano de publicação:
2024
Tipo de documento:
Article