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Functional Characterization of a CruP-Like Isomerase in Dunaliella.
Chen, Hao-Hong; Pang, Xiao-Hui; Dai, Ju-Liang; Jiang, Jian-Guo.
Afiliação
  • Chen HH; College of Food Science and Bioengineering, South China University of Technology, Guangzhou 510640, China.
  • Pang XH; Department of Bioengineering, Imperial College London, London SW7 2AZ, United Kingdom.
  • Dai JL; College of Food Science and Bioengineering, South China University of Technology, Guangzhou 510640, China.
  • Jiang JG; College of Food Science and Bioengineering, South China University of Technology, Guangzhou 510640, China.
J Agric Food Chem ; 72(17): 10005-10013, 2024 May 01.
Article em En | MEDLINE | ID: mdl-38626461
ABSTRACT
Dunaliella bardawil is a marine unicellular green algal that produces large amounts of ß-carotene and is a model organism for studying the carotenoid synthesis pathway. However, there are still many mysteries about the enzymes of the D. bardawil lycopene synthesis pathway that have not been revealed. Here, we have identified a CruP-like lycopene isomerase, named DbLyISO, and successfully cloned its gene from D. bardawil. DbLyISO showed a high homology with CruPs. We constructed a 3D model of DbLyISO and performed molecular docking with lycopene, as well as molecular dynamics testing, to identify the functional characteristics of DbLyISO. Functional activity of DbLyISO was also performed by overexpressing gene in both E. coli and D. bardawil. Results revealed that DbLyISO acted at the C-5 and C-13 positions of lycopene, catalyzing its cis-trans isomerization to produce a more stable trans structure. These results provide new ideas for the development of a carotenoid series from engineered bacteria, algae, and plants.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cis-trans-Isomerases / Liases Intramoleculares / Clorofíceas / Licopeno Idioma: En Revista: J Agric Food Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cis-trans-Isomerases / Liases Intramoleculares / Clorofíceas / Licopeno Idioma: En Revista: J Agric Food Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China