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Morphological-metabolic analysis in Streptomyces rimosus microparticle-enhanced cultivations (MPEC).
Scigaczewska, Anna; Boruta, Tomasz; Bizukojc, Marcin.
Afiliação
  • Scigaczewska A; Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Lodz University of Technology, ul. Wolczanska 213, 93-005, Lodz, Poland. anna.kowalska.1@p.lodz.pl.
  • Boruta T; Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Lodz University of Technology, ul. Wolczanska 213, 93-005, Lodz, Poland.
  • Bizukojc M; Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Lodz University of Technology, ul. Wolczanska 213, 93-005, Lodz, Poland.
Bioprocess Biosyst Eng ; 47(6): 891-902, 2024 Jun.
Article em En | MEDLINE | ID: mdl-38664238
ABSTRACT
Streptomyces produce a broad spectrum of biologically active molecules such as oxytetracycline and rimocidin, which are widely used in human and animal treatments. microparticle-enhanced cultivation (MPEC) is one of the tools used for Streptomyces bioprocesses intensification by the control of mycelial morphology. In the present work, morphological changes of Streptomyces rimosus caused by the addition of 10 µm talc microparticles in MPEC were correlated with the biosynthetic activity of the microorganism. Comparing the runs with and without microparticles, major morphological changes were observed in MPEC, including the deformation of pellets, variation of their size, appearance of hyphae and clumps as well as the aggregation of mycelial objects. The presence of talc microparticles also influenced the levels of the studied secondary metabolites produced by S. rimosus. Comparing control and MPEC runs, the addition of talc microparticles increased the amounts of oxytetracycline (9-fold), 2-acetyl-2-decarboxamido-oxytetracycline (7-fold), milbemycin A3+4[O] (3-fold) and CE 108 (1.5-fold), while rimocidin (27-ethyl) and milbemycin ß11+4[O] production was reduced. In summary, the addition of talc microparticles to S. rimosus cultivations led to the development of smaller morphological forms like hyphae and clumps as well as to the changes in the amounts of secondary metabolites.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Streptomyces rimosus Idioma: En Revista: Bioprocess Biosyst Eng Assunto da revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Polônia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Streptomyces rimosus Idioma: En Revista: Bioprocess Biosyst Eng Assunto da revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Polônia