Phytochemical investigation of Nigella sativa seed extract by HPTLC, HPLC and GC-MS: a comparative geographical study.
Nat Prod Res
; : 1-7, 2024 Apr 29.
Article
em En
| MEDLINE
| ID: mdl-38684035
ABSTRACT
This study aimed to ensure the quality of the seed as well as determine the phytochemical composition of Nigella sativa seed extract (NSSE) obtained from three different geographical locations. Pharmacognostic evaluation of the seed includes preliminary phytochemical screening, physicochemical evaluation, and study of heavy metal content, in addition to HPTLC, HPLC, and GC-MS studies of the extract obtained from the seed of the Nigella sativa (NS). HPTLC fingerprinting studies revealed the presence of various bioactive compounds. HPLC analysis confirms the quantitative variation of thymoquinone (TQ) in the extracts, i.e. the maximum quantity of TQ was found in Vizag NSSE, followed by Punjab and Madhya Pradesh. GC-MS analysis reveals the presence of 33, 35, and 32 constituents in the extract obtained from Vizag, Madhya Pradesh, and Punjab, respectively. This study confirms the variation in the phytochemical composition as well as in the biomarker (Thymoquinone) content present in the collected samples.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Nat Prod Res
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Índia