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Effect of cattle and horse feces storage methods on Nematode egg viability and sensitivity for egg hatch test.
Green de Freitas, Mariana; Gonçalves Lino Borges, Dyego; Henrique Conde, Mário; Takemi Muchon Nakatani, Matheus; Francielle Tutija, Juliane; Fuzaro Scaléa, Giulia Ornellas; Reckziegel, Guilherme Henrique; de Almeida Borges, Fernando.
Afiliação
  • Green de Freitas M; Laboratório de Doenças Parasitárias, Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande - MS, Brazil. Electronic address: marianagreenf@gmail.com.
  • Gonçalves Lino Borges D; Laboratório de Doenças Parasitárias, Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande - MS, Brazil. Electronic address: dyegogborges@hotmail.com.
  • Henrique Conde M; Laboratório de Doenças Parasitárias, Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande - MS, Brazil. Electronic address: marioh.vet@gmail.com.
  • Takemi Muchon Nakatani M; Laboratório de Doenças Parasitárias, Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande - MS, Brazil. Electronic address: matheus_takemi@hotmail.com.
  • Francielle Tutija J; Laboratório de Doenças Parasitárias, Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande - MS, Brazil. Electronic address: juliane_tutija@hotmail.com.
  • Fuzaro Scaléa GO; Laboratório de Doenças Parasitárias, Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande - MS, Brazil. Electronic address: giulia_scalea@hotmail.com.
  • Reckziegel GH; Laboratório de Doenças Parasitárias, Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande - MS, Brazil. Electronic address: ghreckziegel@gmail.com.
  • de Almeida Borges F; Laboratório de Doenças Parasitárias, Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande - MS, Brazil. Electronic address: fernando.borges@ufms.br.
Exp Parasitol ; 262: 108769, 2024 Jul.
Article em En | MEDLINE | ID: mdl-38735519
ABSTRACT
The aim of the present study was to validate methods of stool sample conservation for the egg hatch test (EHT). This study involved the use of a bovine naturally infected predominantly by Cooperia spp. and one equine naturally infected predominantly by cyathostomins characterized as susceptible to benzimidazoles in the EHT. Fecal samples were submitted to three treatments aerobic methods (anaerobic storage in plastic bottles, anaerobic storage in vacuum-sealed bags or aerobic storage in plastic bags), under two temperature conditions (room temperature and refrigeration) analyzed at four different assessment times (48, 72, 96 and 120 h). As the standard test, an assay was also performed within 3 h. The tests were performed in triplicate for each drug concentration and with three experimental repetitions at one-week intervals. Two criteria were used for the storage

methods:

hatchability in the negative control group and sensitivity of the eggs to thiabendazole, comparing the EC50 and 95% confidence interval for each treatment to those of the standard test and the other repetitions. Bovine samples can be stored for up to 96 h and refrigerated vacuum storage can be used, ensuring hatchability of the negative control and sensitivity of the eggs to thiabendazole. For equine samples, no forms of storage were indicated due to the variation among the repetitions and the reduction in the sensitivity of the eggs to thiabendazole, which could result in a false positive detection of resistance.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óvulo / Fezes Limite: Animals Idioma: En Revista: Exp Parasitol Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óvulo / Fezes Limite: Animals Idioma: En Revista: Exp Parasitol Ano de publicação: 2024 Tipo de documento: Article