Development of an Analytical Method for Unsaturated Disaccharides from Heparan Sulfate Using Dansylhydrazine Derivatization and High-Performance Liquid Chromatography with Fluorescence Detection.
Biol Pharm Bull
; 47(5): 1054-1057, 2024.
Article
em En
| MEDLINE
| ID: mdl-38811191
ABSTRACT
Glycosaminoglycans (GAGs), such as heparan sulfate (HS), play essential roles in living organisms. Understanding the functionality of HS and its involvement in disease progression necessitates the sensitive and quantitative detection of HS-derived unsaturated disaccharides. Conventionally, fluorescence derivatization precedes the HPLC analysis of these disaccharides. However, the presence of excess unreacted derivatization reagents can inhibit rapid and sensitive analysis in chromatographic determinations. In this study, we describe analytical methods that use dansylhydrazine as a derivatization agent for the detection and determination of HS-derived unsaturated disaccharides using HPLC. In addition, we have developed a straightforward method for removing excess unreacted reagent using a MonoSpin NH2 column. This method may be employed to remove excess pre-labeling reagents, thereby facilitating the analysis of HS-derived unsaturated disaccharides with satisfactory reproducibility.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Compostos de Dansil
/
Dissacarídeos
/
Heparitina Sulfato
Idioma:
En
Revista:
Biol Pharm Bull
/
Biol. pharm. bull
/
Biological and pharmaceutical bulletin
Assunto da revista:
BIOQUIMICA
/
FARMACOLOGIA
Ano de publicação:
2024
Tipo de documento:
Article