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Omaveloxolone ameliorates glucocorticoid-induced osteonecrosis of the femoral head by promoting osteogenesis and angiogenesis.
Wan, Changtao; Liang, Chuancai; Peng, Hao.
Afiliação
  • Wan C; Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China; Department of Orthopedics, The Third People's Hospital of Hubei Province, Wuhan, China.
  • Liang C; Department of Emergency, Wuhan University Renmin Hospital, Wuhan, China. Electronic address: 2020283020228@whu.edu.cn.
  • Peng H; Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China. Electronic address: penghao8599@163.com.
Biochem Biophys Res Commun ; 723: 150188, 2024 09 03.
Article em En | MEDLINE | ID: mdl-38824808
ABSTRACT
Steroid (glucocorticoid)-induced necrosis of the femoral head (SONFH) represents a prevalent, progressive, and challenging bone and joint disease characterized by diminished osteogenesis and angiogenesis. Omaveloxolone (OMA), a semi-synthetic oleanocarpane triterpenoid with antioxidant, anti-inflammatory, and osteogenic properties, emerges as a potential therapeutic agent for SONFH. This study investigates the therapeutic impact of OMA on SONFH and elucidates its underlying mechanism. The in vitro environment of SONFH cells was simulated by inducing human bone marrow mesenchymal stem cells (hBMSCs) and human umbilical vein endothelial cells (HUVECs) using dexamethasone (DEX).Various assays, including CCK-8, alizarin red staining, Western blot, qPCR, immunofluorescence, flow cytometry, and TUNNEL, were employed to assess cell viability, STING/NF-κB signaling pathway-related proteins, hBMSCs osteogenesis, HUVECs migration, angiogenesis, and apoptosis. The results demonstrate that OMA promotes DEX-induced osteogenesis, HUVECs migration, angiogenesis, and anti-apoptosis in hBMSCs by inhibiting the STING/NF-κB signaling pathway. This experimental evidence underscores the potential of OMA in regulating DEX-induced osteogenesis, HUVECs migration, angiogenesis, and anti-apoptosis in hBMSCs through the STING/NF-κB pathway, thereby offering a promising avenue for improving the progression of SONFH.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteogênese / Neovascularização Fisiológica / Necrose da Cabeça do Fêmur / Glucocorticoides Limite: Humans Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteogênese / Neovascularização Fisiológica / Necrose da Cabeça do Fêmur / Glucocorticoides Limite: Humans Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China