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Membrane nanotubes transform into double-membrane sheets at condensate droplets.
Zhao, Ziliang; Satarifard, Vahid; Lipowsky, Reinhard; Dimova, Rumiana.
Afiliação
  • Zhao Z; Max Planck Institute of Colloids and Interfaces, Potsdam 14476, Germany.
  • Satarifard V; Leibniz Institute of Photonic Technology e.V., Jena 07745, Germany.
  • Lipowsky R; Institute of Applied Optics and Biophysics, Friedrich-Schiller-University Jena, Jena 07743, Germany.
  • Dimova R; Max Planck Institute of Colloids and Interfaces, Potsdam 14476, Germany.
Proc Natl Acad Sci U S A ; 121(26): e2321579121, 2024 Jun 25.
Article em En | MEDLINE | ID: mdl-38900795
ABSTRACT
Cellular membranes exhibit a multitude of highly curved morphologies such as buds, nanotubes, cisterna-like sheets defining the outlines of organelles. Here, we mimic cell compartmentation using an aqueous two-phase system of dextran and poly(ethylene glycol) encapsulated in giant vesicles. Upon osmotic deflation, the vesicle membrane forms nanotubes, which undergo surprising morphological transformations at the liquid-liquid interfaces inside the vesicles. At these interfaces, the nanotubes transform into cisterna-like double-membrane sheets (DMS) connected to the mother vesicle via short membrane necks. Using super-resolution (stimulated emission depletion) microscopy and theoretical considerations, we construct a morphology diagram predicting the tube-to-sheet transformation, which is driven by a decrease in the free energy. Nanotube knots can prohibit the tube-to-sheet transformation by blocking water influx into the tubes. Because both nanotubes and DMSs are frequently formed by cellular membranes, understanding the formation and transformation between these membrane morphologies provides insight into the origin and evolution of cellular organelles.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polietilenoglicóis / Nanotubos Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polietilenoglicóis / Nanotubos Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Alemanha