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Lipidomics of homeoviscous adaptation to low temperatures in Staphylococcus aureus utilizing exogenous straight-chain unsaturated fatty acids.
Barbarek, Shannon C; Shah, Ritika; Paul, Sharanya; Alvarado, Gloria; Appala, Keerthi; Phillips, Caiden; Henderson, Emma C; Strandquist, Evan T; Pokorny, Antje; Singh, Vineet K; Gatto, Craig; Dahl, Jan-Ulrik; Hines, Kelly M; Wilkinson, Brian J.
Afiliação
  • Barbarek SC; School of Biological Sciences, Illinois State University, Normal, Illinois, USA.
  • Shah R; School of Biological Sciences, Illinois State University, Normal, Illinois, USA.
  • Paul S; School of Biological Sciences, Illinois State University, Normal, Illinois, USA.
  • Alvarado G; School of Biological Sciences, Illinois State University, Normal, Illinois, USA.
  • Appala K; Department of Chemistry, University of Georgia, Athens, Georgia, USA.
  • Phillips C; Department of Microbiology and Immunology, Kirksville College of Osteopathic Medicine, A. T. Still University of Health Sciences, Kirksville, Missouri, USA.
  • Henderson EC; School of Biological Sciences, Illinois State University, Normal, Illinois, USA.
  • Strandquist ET; School of Biological Sciences, Illinois State University, Normal, Illinois, USA.
  • Pokorny A; Department of Chemistry and Biochemistry, University of North Carolina-Wilmington, Wilmington, North Carolina, USA.
  • Singh VK; Department of Microbiology and Immunology, Kirksville College of Osteopathic Medicine, A. T. Still University of Health Sciences, Kirksville, Missouri, USA.
  • Gatto C; School of Biological Sciences, Illinois State University, Normal, Illinois, USA.
  • Dahl J-U; School of Biological Sciences, Illinois State University, Normal, Illinois, USA.
  • Hines KM; Department of Chemistry, University of Georgia, Athens, Georgia, USA.
  • Wilkinson BJ; School of Biological Sciences, Illinois State University, Normal, Illinois, USA.
J Bacteriol ; 206(7): e0018724, 2024 07 25.
Article em En | MEDLINE | ID: mdl-38953643
ABSTRACT
It is well established that Staphylococcus aureus can incorporate exogenous straight-chain unsaturated fatty acids (SCUFAs) into membrane phospho- and glyco-lipids from various sources in supplemented culture media and when growing in vivo during infection. Given the enhancement of membrane fluidity when oleic acid (C181Δ9) is incorporated into lipids, we were prompted to examine the effect of medium supplementation with C181Δ9 on growth at low temperatures. C181Δ9 supported the growth of a cold-sensitive, branched-chain fatty acid (BCFA)-deficient mutant at 12°C. Interestingly, we found similar results in the BCFA-sufficient parental strain, supported by the fact that the incorporation of C181Δ9 into the membrane increased membrane fluidity in both strains. We show that the incorporation of C181Δ9 and its elongation product C201Δ11 into membrane lipids was required for growth stimulation and relied on a functional FakAB incorporation system. Lipidomics analysis of the phosphatidylglycerol and diglycosyldiacylglycerol lipid classes revealed major impacts of C181Δ9 and temperature on lipid species. Growth at 12°C in the presence of C181Δ9 also led to increased production of the carotenoid pigment staphyloxanthin. The enhancement of growth by C181Δ9 is an example of homeoviscous adaptation to low temperatures utilizing an exogenous fatty acid. This may be significant in the growth of S. aureus at low temperatures in foods that commonly contain C181Δ9 and other SCUFAs in various forms. IMPORTANCE We show that Staphylococcus aureus can use its known ability to incorporate exogenous fatty acids to enhance its growth at low temperatures. Individual species of phosphatidylglycerols and diglycosyldiacylglycerols bearing one or two degrees of unsaturation derived from the incorporation of C181Δ9 at 12°C are described for the first time. In addition, enhanced production of the carotenoid staphyloxanthin occurs at low temperatures. The studies describe a biochemical reality underlying membrane biophysics. This is an example of homeoviscous adaptation to low temperatures utilizing exogenous fatty acids over the regulation of the biosynthesis of endogenous fatty acids. The studies have likely relevance to food safety in that unsaturated fatty acids may enhance the growth of S. aureus in the food environment.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Staphylococcus aureus / Adaptação Fisiológica / Temperatura Baixa / Ácidos Graxos Insaturados / Lipidômica Idioma: En Revista: J Bacteriol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Staphylococcus aureus / Adaptação Fisiológica / Temperatura Baixa / Ácidos Graxos Insaturados / Lipidômica Idioma: En Revista: J Bacteriol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos