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Protocol to electroporate DNA plasmids into Ciona robusta embryos at the 1-cell stage.
Jindal, Granton A; Lim, Fabian; Tellez, Krissie; Song, Benjamin P; Bantle, Alexis T; Farley, Emma K.
Afiliação
  • Jindal GA; Department of Medicine, Health Sciences, University of California, San Diego, La Jolla, CA 92093, USA; Department of Molecular Biology, Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: gajindal@gmail.com.
  • Lim F; Department of Medicine, Health Sciences, University of California, San Diego, La Jolla, CA 92093, USA; Department of Molecular Biology, Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA; Biological Sciences Graduate Program, University of California, San Diego, La Jol
  • Tellez K; Department of Medicine, Health Sciences, University of California, San Diego, La Jolla, CA 92093, USA; Department of Molecular Biology, Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Song BP; Department of Medicine, Health Sciences, University of California, San Diego, La Jolla, CA 92093, USA; Department of Molecular Biology, Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA; Biological Sciences Graduate Program, University of California, San Diego, La Jol
  • Bantle AT; Department of Medicine, Health Sciences, University of California, San Diego, La Jolla, CA 92093, USA; Department of Molecular Biology, Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA; Biological Sciences Graduate Program, University of California, San Diego, La Jol
  • Farley EK; Department of Medicine, Health Sciences, University of California, San Diego, La Jolla, CA 92093, USA; Department of Molecular Biology, Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: efarley@ucsd.edu.
STAR Protoc ; 5(3): 103107, 2024 Jul 03.
Article em En | MEDLINE | ID: mdl-38963758
ABSTRACT
Electroporation is a technique to introduce DNA constructs into cells using electric current. Here, we present a protocol to electroporate DNA plasmids into Ciona robusta embryos at the 1-cell stage. We describe steps for setting up and conducting electroporation. We then detail procedures for collecting, fixing, and mounting embryos and counting expression. This protocol can be used to study the expression of enhancers via reporter assays, manipulating cells using genes or modified genes such as dominant negatives, and genome editing. For complete details on the use and execution of this protocol, please refer to Song, et al.1.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: STAR Protoc Ano de publicação: 2024 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: STAR Protoc Ano de publicação: 2024 Tipo de documento: Article