Infection of Culicoides Biting Midges with Epizootic Hemorrhagic Disease Virus.
Methods Mol Biol
; 2838: 221-237, 2024.
Article
em En
| MEDLINE
| ID: mdl-39126636
ABSTRACT
Epizootic hemorrhagic disease virus (EHDV) is transmitted by Culicoides biting midges. Studies aiming to predict the likely spread of EHDV require an understanding of the viral infection and replication kinetics within these insects, including the proportion of the insect population that are able to support virus transmission. Here, we describe methods for the infection of Culicoides with EHDV in the laboratory via oral infection using an artificial membrane system or a cotton pledget and intrathoracic (IT) inoculation. Each method can be used to explore determinants of vector competence of Culicoides species and populations for EHDV.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ceratopogonidae
/
Vírus da Doença Hemorrágica Epizoótica
/
Infecções por Reoviridae
/
Insetos Vetores
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Reino Unido