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In vitro characterization of probiotic potential of Lactobacillus plantarum CM49 against selected cattle mastitogens.
Izhar, Muhammad Zeeshan; Nawaz, Muhammad; Yaqub, Tahir; Avais, Muhammad; Anjum, Aftab Ahmad.
Afiliação
  • Izhar MZ; Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan.
  • Nawaz M; Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan. muhammad.nawaz@uvas.edu.pk.
  • Yaqub T; Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan.
  • Avais M; Department of Medicine, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan.
  • Anjum AA; Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan.
BMC Microbiol ; 24(1): 310, 2024 Aug 22.
Article em En | MEDLINE | ID: mdl-39174931
ABSTRACT

BACKGROUND:

Bovine mastitis results in significant economic losses for the dairy industry globally due to milk production losses and decreased herd efficiency. This research aimed to isolate, select, and characterize indigenous lactobacilli with probiotic properties. A total of 40 lactobacilli were isolated from healthy milk samples of cattle and identified at the species level through 16S rDNA sequencing. All isolates were initially screened for antimicrobial activity, and selected isolates underwent in vitro assessment of probiotic properties.

RESULTS:

Among the lactobacilli isolates, varying levels of activity (9 to 19 mm) against cattle mastitogens; Stapylococcus aureus (Staph. aureus), Escherichia coli (E. coli) and Streptococcus dysgalactiae (Strep. dysgalactiae) were observed in the well diffusion assay. These isolates demonstrated auto-aggregation (ranging from 14.29 ± 0.96% to 62.11 ± 1.09%) and co-aggregate (ranging from 9.21 ± 0.14% to 55.74 ± 0.74%) with mastitogens after 2 h. Lactobacillus (Lb.) plantarum CM49 showed sensitivity to most antibiotics tested and exhibited strong inhibitory effects, with mean log10 reductions of 3.46 for Staph. aureus, 2.82 for E. coli, and 1.45 for Strep. dysgalactiae in co-culture experiments. Furthermore, Lb. plantarum CM49 significantly decreased the adhesion rate of mastitogens on the bovine mammary cell line and mouse model, demonstrating its potential effectiveness in preventing mastitis.

CONCLUSION:

It is concluded that Lb. plantarum CM49 has remarkable probiotic potential with activity against cattle mastitogens in the laboratory and cell culture and competitively excludes mastitogens from bovine mammary cells and ameliorates Staph. aureus-induced mastitis in mice.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Staphylococcus aureus / Probióticos / Leite / Lactobacillus plantarum / Escherichia coli / Mastite Bovina Limite: Animals Idioma: En Revista: BMC Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Paquistão

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Staphylococcus aureus / Probióticos / Leite / Lactobacillus plantarum / Escherichia coli / Mastite Bovina Limite: Animals Idioma: En Revista: BMC Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Paquistão