Concentration of viable spermatozoa for artificial insemination.
Fertil Steril
; 45(1): 132-4, 1986 Jan.
Article
em En
| MEDLINE
| ID: mdl-3943644
ABSTRACT
Glass wool microfiber code 112 (approximately 30 mg) was gently packed to a depth of 3 mm in a 3-ml disposable syringe barrel. The column was rinsed repeatedly with Tyrode solution to remove loose glass wool fibers. Approximately 0.3 to 0.5 ml of concentrated spermatozoa (after centrifugation of semen at 500 X g for 5 minutes) was layered over the wet column and allowed to filter by gravity. The filtered spermatozoa demonstrated a significantly higher (P less than 0.001) mean percentage of motility, progressive motility, sperm unstained by eosin Y dye, and sperm with functionally intact membranes. Although there was a considerable loss of sperm, the filtered specimen contained all viable spermatozoa present before filtration. Therefore, it appears that this procedure yields a high percentage of viable spermatozoa, potentially capable of fertilization, for use in in vitro fertilization or, possibly, artificial insemination.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espermatozoides
/
Inseminação Artificial
Limite:
Humans
/
Male
Idioma:
En
Revista:
Fertil Steril
Ano de publicação:
1986
Tipo de documento:
Article