The effect of transferrin saturation on the estimation of erythropoietin by the mouse spleen cell microassay.
Exp Hematol
; 13(10): 1007-13, 1985 Nov.
Article
em En
| MEDLINE
| ID: mdl-4054239
ABSTRACT
The effect of transferrin from various sources and the degree of saturation with iron on the stimulation of DNA synthesis by erythropoietin (Epo) has been investigated. Mouse, human, and bovine transferrins saturated with iron caused an increase in thymidine incorporation both in the absence and presence of Epo. In contrast, exogenous human and bovine apotransferrin resulted in significantly decreased incorporation of the tracer. The iron saturation of serum alters its apparent erythropoietic activity. This transferrin saturation effect may be overcome by a simple modification involving the addition of iron to the culture medium.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transferrina
/
Linfócitos
/
Eritropoetina
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Exp Hematol
Ano de publicação:
1985
Tipo de documento:
Article