A micro-technique for preparing homogenates of biological samples.
Anal Biochem
; 151(2): 400-2, 1985 Dec.
Article
em En
| MEDLINE
| ID: mdl-4096377
ABSTRACT
A method is described for preparing homogenates of insects, mites, nematodes, or fungal spores for electrophoretic analysis which overcomes the disadvantages of existing methods. It is also applicable to the homogenization of microsamples of animal tissue. The method involves grinding the samples in glass tubes, closed at one end, prepared from melting-point capillaries. A range of plungers is fabricated from the stainless-steel plungers from broken microsyringes, or from stainless-steel wire. The plunger is operated by hand to homogenize a sample in 2-20 microliter of buffer in a tube. The techniques for handling the samples before and after homogenization and for labeling and centrifuging them are described. Compared with existing methods, the procedure minimizes sample loss and risk of cross-contamination and eliminates the possibility of overheating the sample during homogenization.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas
Limite:
Animals
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
1985
Tipo de documento:
Article