Lipid carriers in the synthesis of high-mannose glycoproteins in algae.
Biochim Biophys Acta
; 586(3): 545-59, 1979 Sep 03.
Article
em En
| MEDLINE
| ID: mdl-476152
ABSTRACT
Particulate preparations from the chlorophyta Prototheca zopfii catalyze the incorporation of mannose and N-acetylglucosamine into glycolipids. These had been characterized as lipid monophosphate mannose, lipid pyrophosphate N,N'-diacetylchitobiose and various lipid-linked oligosaccharides containing two N-acetylglucosamine residues plus a variable number of mannose residues. The lipid moiety has the properties expected for dolichyl phosphate. The oligosacchride-linked lipids serve as precursors for the formation of a polymer sensible to pronase digestion. The oligosaccharide is linked by N-glycosidic linkage to an asparagine residue. In longer incubation periods, a polymer insensitive to pronase hydrolysis, but precipitable by copper salts such as cell wall mannans is formed. Polymer formation is inhibited by 1 mM bacitracin. The reactions leading to the formation of the mannoprotein were found associated to the rough endoplasmic reticulum. The synthesis of mannans was found to occur in the Golgi vesicles.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glicolipídeos
/
Glicoproteínas
/
Clorófitas
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1979
Tipo de documento:
Article